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目的 研究缺氧诱导因子-1在人乳腺癌细胞系MCF-7细胞中对凋亡的作用.方法 氯化钴(COCl2)模拟低氧环境.采用RNA干扰技术.构建针对HIF-1α的shRNA真核表达栽体,转染MCF-7细胞.Real-time PCR和Western-blot技术分别检测HIF-1αmRNA和蛋白质水平.Sub-G1测定,AnnexinV荧光标记和DNA ladder检测细胞凋亡.结果 缺氧后MCF-7细胞HIF-1α的蛋白质水平表达增加,但缺氧后细胞的凋亡率比正常氧水平时低.实时定量PCR和Western blot结果证实转染短发夹RNA后,MCF-7细胞中HIF-1α基因表达被成功抑制.阿糖胞苷诱导或无凋亡诱导剂时,干扰组凋亡率比未转染组明显增高.结论 这项研究证明HIF-1在MCF-7中发挥抗细胞凋亡的作用.为针对HIF-1α的shRNA有效地用于乳腺癌的治疗提供新的思路.“,”HIF-1 (hypoxia-inducible factor-1) is the major transcriptional factor responsible for oxygen-dependent gene expression.Suppression of HIF-1α is important for exploring HIF-1-dependent pathophysiological processes. In this study, we investigated the effect of short hairpin RNA (shRNA targeting HIF-1α on the human breast carcinoma MCF-7 cell line. The protein level of HIF-1α increased after exposure of MCF-7 cells to hypoxia. However,apoptosis was lower in hypoxia compared with normoxia. The results of real-time PCR and western blot validated that the HIF-1α gene of MCF-7 cells transfected with HIF-1α shRNA was suppressed successfully. When the cells were treated with or without apoptosis inducer Ara-c ,the apoptosis rate of MCF-7 cells transfected with HIF-1α shRNA was significantly higher than that of untransfected cells. Then the study proves the anti-apoptotic role of HIF-1 in MCF-7 breast cancer cell line and brings new insight into the strategy of using shRNA targeting HIF-1α to treat breast cancer.