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利巴韦林具有广谱抗病毒作用,甘草酸具有抗炎、免疫调节和抗病毒活性。为了增强抗病毒药效、减少利巴韦林的不良反应,本文对二者联合应用的抗流感病毒效果进行了研究。以H1N1亚型流感病毒人工感染BALB/c小鼠,建立小鼠病毒性肺炎模型。以小鼠存活率、肺指数抑制率和肺病毒滴度等为评价指标,考察甘草酸和利巴韦林联用对感染H1N1亚型流感病毒小鼠的保护作用。结果表明,甘草酸和利巴韦林联用能显著抑制H1N1亚型流感病毒引起的小鼠肺炎实变,对攻毒后第5天小鼠肺指数抑制率为36%;两药联用对感染小鼠有协同保护作用,甘草酸(50 mg·kg-1·d-1)与利巴韦林(40 mg·kg-1·d-1)联用对感染小鼠的保护率达到100%,显著高于病毒对照组和单一药物组(P<0.01),协同值为36。两药联用显著降低感染小鼠肺组织的病毒滴度(P<0.01),同时对病毒感染诱导的小鼠血浆炎症因子IL-6(P<0.01)、TNF-α(P<0.01)和IL-1β(P<0.05)的升高具有显著抑制作用。结果提示,甘草酸和利巴韦林联用对感染H1N1亚型流感病毒小鼠具有协同保护作用,在临床上具有重要的应用价值。
Ribavirin has a broad spectrum of antiviral activity and glycyrrhizin has anti-inflammatory, immunomodulatory and antiviral activities. In order to enhance the antiviral efficacy and reduce the adverse reactions of ribavirin, the anti-influenza virus effect of the combination of the two was studied in this paper. BALB / c mice were infected with H1N1 subtype influenza virus to establish mouse model of viral pneumonia. The survival rate of mice, the inhibition rate of lung index and the titer of lung virus were used as evaluation indexes to investigate the protective effect of glycyrrhizin and ribavirin on mice infected with H1N1 subtype influenza virus. The results showed that combination of glycyrrhizin and ribavirin could significantly inhibit the pneumonia in mice induced by H1N1 subtype influenza virus, and the lung index inhibition rate was 36% on the fifth day after challenge in mice; Infected mice had a synergistic protective effect. The protection of glycyrrhizin (50 mg · kg-1 · d-1) and ribavirin (40 mg · kg-1 · d-1) %, Significantly higher than the virus control group and single drug group (P <0.01), the synergistic value of 36. The combination of the two drugs significantly reduced the virus titer in the lung tissue of infected mice (P <0.01). At the same time, the inflammatory factors IL-6 (P <0.01), TNF-α IL-1β (P <0.05) had a significant inhibitory effect. The results suggest that combination of glycyrrhizin and ribavirin has synergistic protective effect on mice infected with H1N1 subtype influenza virus and has important clinical value.