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【目的】观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体(sTRAILR1和sTRAILR4)在毛细支气管炎和急性上呼吸道感染患儿中的表达,探讨它们在呼吸道感染炎症机制中的作用。【方法】ELISA法检测毛细支气管炎和急性上呼吸道感染患儿及健康对照组儿童血清sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度。【结果】毛细支气管炎组和急性上呼吸道感染组患者血清中sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度分别为(85±38)、(71±19)、(167±97);(78±28)、(61±15)、(139±72)pg/mL均显著高于对照组(55±19)、(45±14)、(56±38)pg/mL(P均<0.01);毛细支气管炎组sTRAILR1的蛋白浓度显著高于急性上呼吸道感染组(P<0.05),两组sTRAIL和sTRAILR4的蛋白浓度相比差异无统计学意义(P均>0.05)。sTRAILR1分别与sTRAIL、sTRAILR4的蛋白浓度呈显著正相关(n=102,r分别为0.236和0.409,P均<0.01)。【结论】毛细支气管炎和急性上呼吸道感染患儿血清中可溶性TRAIL及其受体TRAILR1和TRAILR4的表达增加,它们可能参与了呼吸道感染的炎症过程,TRAIL可能主要通过TRAILR1起作用,它们的表达水平增高可能被视为病情活动的指标之一。
【Objective】 To observe the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its receptors (sTRAILR1 and sTRAILR4) in children with bronchiolitis and acute upper respiratory tract infection, and to explore their roles in inflammation of respiratory tract infections . 【Methods】 Serum levels of sTRAIL, sTRAILR1 and sTRAILR4 in children with bronchiolitis and acute upper respiratory tract infection and healthy controls were detected by ELISA. 【Results】 Serum levels of sTRAIL, sTRAILR1 and sTRAILR4 in patients with bronchiolitis and acute upper respiratory tract infection were (85 ± 38), (71 ± 19), (167 ± 97), (78 ± 28), (61 ± 15) and (139 ± 72) pg / mL were significantly higher than that of the control group (55 ± 19), (45 ± 14) and (56 ± 38) pg / mL The protein concentration of sTRAILR1 was significantly higher than that of acute upper respiratory tract infection (P <0.05). There was no significant difference in sTRAIL and sTRAILR4 protein concentrations between the two groups (all P> 0.05). sTRAILR1 was significantly and positively correlated with the protein concentration of sTRAIL and sTRAILR4 (n = 102, r = 0.236 and 0.409, respectively, P <0.01). 【Conclusions】 Serum levels of soluble TRAIL and its receptors TRAILR1 and TRAILR4 are increased in children with bronchiolitis and acute upper respiratory tract infection, which may be involved in the inflammatory process of respiratory tract infection. TRAIL may play a role mainly through TRAILR1 and their expression levels Elevated may be considered one of the indicators of disease activity.