论文部分内容阅读
目的探讨转化生长因子-β1(TGF-β1)基因的多态性与人类长寿的相关性。方法采用聚合酶链式反应-限制性片段长度多态性技术,检测198例广西巴马地区健康壮族长寿老人和177例巴马地区健康壮族成年人TGF-β1基因的G-800A、C-509T、T+869C、G+915C多态位点,并比较两组人群TGF-β1基因的相应基因型频率和等位基因频率。结果研究对象中均未检测出G-800A、G+915C多态位点,G-800A位点的基因型均为-800GG,G+915C位点的基因型均为+915GG。C-509T和T+869C两位点均存在多态性。长寿组的等位基因-509C和+869T的频率均明显高于对照组(分别为27.78%和20.90%,P=0.029;35.10%和27.97%,P=0.036)。长寿组基因型为-509CT和+869TT的频率均明显高于对照组(分别为37.37%和24.29%,P=0.006;20.20%和10.17%,P=0.007),可能为长寿的有利因素(OR分别为1.86和2.24);而-509TT的频率明显低于对照组(分别为53.54%和67.23%,P=0.007),可能是长寿的不利因素(OR=0.56)。在G-800A、C-509T、T+869C、G+915C多态位点基因型组合中,长寿组和对照组出现频率最高的均为GG-TT-CC-GG,但无显著性差异(分别为45.45%和52.54%,P=0.170);长寿组GG-CT-TT-GG的频率明显高于对照组(分别为11.11%和1.13%,P=0.000),是长寿的有利因素(OR=10.94)。结论在巴马人群中,TGF-β1基因内带有-509CT和+869TT基因型的个体可能有利于长寿,而-509TT基因型可能是长寿的不利因子。
Objective To investigate the relationship between the polymorphism of transforming growth factor-β1 (TGF-β1) gene and human longevity. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the expression of G-800A and C-509T in TGF-β1 gene in 198 healthy Zhuang longevity elderly in Bama region and 177 healthy adults in Bama region of China. , T + 869C, G + 915C polymorphism loci, and compared the corresponding genotype frequency and allele frequency of TGF-β1 gene in two groups of people. Results No G-800A, G + 915C polymorphic loci were detected in the study subjects. The genotype of G-800A locus was -800GG, and the genotype of G +915C locus was +915GG. Polymorphism existed in both loci C-509T and T + 869C. The frequencies of alleles -509C and + 869T in the longevity group were significantly higher than those in the control group (27.78% and 20.90%, respectively; P = 0.029; 35.10% and 27.97%; P = 0.036). The frequencies of -509CT and + 869TT genotypes in longevity group were significantly higher than those in control group (37.37% and 24.29%, respectively; P = 0.006; 20.20% and 10.17%, P = 0.007) (1.86 and 2.24, respectively). However, the frequency of -509TT was significantly lower than that of the control group (53.54% and 67.23%, respectively, P = 0.007), which may be the unfavorable factor for longevity (OR = 0.56). In G-800A, C-509T, T + 869C and G + 915C genotype combinations, GG-TT-CC-GG was the most frequent genotype in the longevity group and the control group, but no significant difference was found (45.45% and 52.54%, P = 0.170, respectively). The frequency of GG-CT-TT-GG in longevity group was significantly higher than that in control group (11.11% and 1.13%, P = 0.000) = 10.94). Conclusion In the Bama population, individuals with the -509CT and + 869TT genotypes within the TGF-β1 gene may be beneficial for longevity, while the -509TT genotype may be a negative factor for longevity.