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目的探讨中心体α-微管蛋白和γ-微管蛋白在脑胶质瘤中的表达,及其与凋亡抑制基因Survivin表达的相关性。方法应用免疫组织化学方法检测51例脑胶质瘤组织中α-微管蛋白、Survivin蛋白的表达,免疫荧光染色方法检测γ-微管蛋白的表达,并分析其之间的相关性。结果脑胶质瘤高级别组(Ⅲ~Ⅳ级)α-微管蛋白、γ-微管蛋白及Survivin蛋白表达阳性率(88.0%、80.0%、92.0%)明显高于低级别组(Ⅰ、Ⅱ级)(22.2%、47.1%;11.1%、35.3%;33.3%、52.9%),且差异具有统计学意义(P均<0.0125)。α-微管蛋白、γ-微管蛋白与Survivin表达之间均呈正相关(r分别为0.440 40、.547 8,P<0.05)。结论α-微管蛋白、γ-微管蛋白和Survivin在胶质瘤中的表达随恶性程度升高而增加,且相互之间均呈正相关。微管蛋白与Survivin的相互作用可能是胶质瘤发生发展过程中的重要事件。
Objective To investigate the expression of central α-tubulin and γ-tubulin in gliomas and its correlation with the expression of Survivin. Methods The expressions of α-tubulin and Survivin in 51 glioma tissues were detected by immunohistochemistry. The expression of γ-tubulin was detected by immunofluorescence staining and the correlation was analyzed. Results The positive rates of α-tubulin, γ-tubulin and Survivin protein in high grade glioma group (Ⅲ ~ Ⅳ grade) were significantly higher than those in lower grade group (Ⅰ, Grade Ⅱ) (22.2%, 47.1%; 11.1%, 35.3%; 33.3%, 52.9% respectively), with statistical significance (all P <0.0125). α-tubulin, γ-tubulin and Survivin expression were positively correlated (r = 0.440 40, .547 8, P <0.05). Conclusion The expression of α-tubulin, γ-tubulin and Survivin in gliomas increased with the increase of malignant degree, and they were positively correlated with each other. The interaction between tubulin and Survivin may be an important event in the development of glioma.