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目的 :研究大鼠脑出血前脑内抑凋亡基因bcl- 2的表达 ,以及三七总皂甙对其表达的影响。方法 :用原位杂交法观察胶原酶诱导的脑出血大鼠前脑内bcl- 2mRNA在对照组和给药组的表达变化 ;用免疫组化法观察Bcl- 2蛋白在对照组和给药组的表达变化。结果 :bcl- 2mRNA及Bcl - 2蛋白主要表达于病灶及病灶周围、大脑皮质额叶、顶叶、梨状区皮质、海马CA1至CA4区 ,给药组阳性细胞数量显著增加 ,细胞着色变深 ;将给药组与对照组Bcl- 2蛋白、bcl- 2mRNA在病灶周围、皮层、海马处阳性细胞的面密度进行图像分析并统计 ,结果P<0 .0 1,具有统计学意义。结论 :脑出血大鼠前脑内抑凋亡基因bcl- 2的转录及蛋白表达水平均上调 ;三七总皂甙促进bcl- 2mRNA的转录与Bcl- 2蛋白的表达 ,减少细胞凋亡 ,促进脑出血后前脑内神经元的存活及损伤修复。
Objective : To study the expression of bcl-2, a suppressor of apoptosis in brain before intracerebral hemorrhage in rats, and the effect of Panax notoginseng saponins on its expression. METHODS: The expression of bcl-2 mRNA in the forebrain of rats with collagenase-induced intracerebral hemorrhage was observed by in situ hybridization. The expression of Bcl-2 protein in the control and administration groups was observed by immunohistochemistry. The expression changes. RESULTS: Bcl-2 mRNA and Bcl-2 protein were mainly expressed in lesions and around the lesion, cortex in frontal lobe, parietal lobe, coronal cortex, and hippocampal CA1 to CA4 regions. The number of positive cells in the administration group was significantly increased, and cells were stained darker. The area density of positive cells of Bcl-2 protein and bcl-2 mRNA around the lesion, cortex, and hippocampus in the administration group and the control group was analyzed and statistically analyzed, and the result was statistically significant (P<0.01). Conclusion: The transcription and protein expression of apoptotic suppressor gene bcl-2 in the forebrain of rats with intracerebral hemorrhage are up-regulated. Panax notoginseng can promote the transcription of bcl-2 mRNA and the expression of Bcl-2 protein, reduce the apoptosis, and promote the brain Neuronal survival and injury repair in the forebrain after hemorrhage.