菲立磁标记胚胎干细胞适宜示踪浓度的研究

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目的 探讨适宜体内移植示踪研究的胚胎干细胞(ESCs)菲立磁标记浓度.方法 分别以0.05、0.10 g/L硫酸鱼精蛋白-菲立磁复合物标记胚胎干细胞,行电镜检测及MRI扫描了解菲立磁标记细胞体外生物学特征.结果 0.05 g/L浓度的硫酸鱼精蛋白-菲立磁复合物标记胚胎干细胞效率最高,达到91.5%以上,标记细胞数量为1×105时能有效引发MRI信号改变.结论 以0.05 g/L硫酸鱼精蛋白-菲立磁复合物浓度标记胚胎干细胞可以作为胚胎干细胞体内移植适宜的标记浓度.
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