Protective Effect of Irbesartan on ATP Binding Cassette Transporter A1 in THP-1 Derived Macrophages

• 作 者: 张慧玲 李清贤 王彦富 石胜伟
• 机 构: The Affiliated Hospital of Jining Medical College,
• 出 处: South China Journal of Cardiology
• 发表日期: 2009年04期
• 关键词: cholesterol protective alteration Electron protect incubation embedding sections

Objectives To explore the protective effect of irbesartan(Irb) under the interference with angiotensinⅡ(AngⅡ) on ABCA1.Methods Electron microscopy was used to detect the morphous of foam cells.The expression of ABCA1 mRNA and its protein were determined by RT-PCR and Western blotting,respectively.The variance of cellular cholesterol content was measured by zymochemistry via-fluorospectrophotometer.Results A positive facilitative effect of AngⅡon the formation of foam cells was observed.Total cholesterol was increased significantly by AngⅡ,the expression of ABCA1 was down-regulated obviously by AngⅡ;Irb could protect ABCA1 against the lesion of AngⅡ;Total cellular cholesterol content was reduced significantly in Irb + AngⅡgroup;However,considerable alteration about the cholesterol content and the expression of ABCA1 were not observed in Irb group without incubation with AngⅡ.Conclusions Irb could protect ABCA1 against the lesion of AngⅡ,which may contribute to its anti-atherosclerotic properties.

Objectives To explore the protective effect of irbesartan (Irb) under the interference with angiotensinⅡ (AngⅡ) on ABCA1.Methods Electron microscopy was used to detect the morphous of foam cells. The expression of ABCA1 mRNA and its protein were determined by RT-PCR and Western blotting, respectively. The variance of cellular cholesterol content was measured by zymochemistry via-fluorospectrophotometer. Results A positive facilitative effect of Ang IIon the formation of foam cells was observed. Total cholesterol was increased significantly by Ang II, the expression of ABCA1 was down-regulated obviously by Ang II; Irb could protect ABCA1 against the lesion of Ang II; Total cellular cholesterol content was reduced significantly in Irb + AngⅡgroup; However, considerable alteration about the cholesterol content and the expression of ABCA1 were not observed in Irb group without incubation with Ang II. Conclusions Irb could protect ABCA1 against the lesion of Ang II, which may contribute to its anti -atherosclerotic properties.