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目的:中枢神经系统中神经干细胞间缝隙连接通讯在神经发生、细胞迁移等方面起着重要作用,而作为电突触的缝隙连接易受到电磁力学的影响。实验拟研究磁场对大鼠胚胎脊髓神经干细胞缝隙连接的作用。方法:实验于2004-01/10在第三军医大学野战外科研究所完成。应用13E大鼠脊髓神经干细胞离体培养,同时应用磁场方向与培养皿底面垂直的恒定磁场处理。在激光共聚焦显微镜下,观察缝隙连接蛋白32(Connexin32,Cx32)的变化。结果:显微镜下,没有磁场处理的脊髓神经干细胞Cx32表达非常明显,而在同样时期培养神经干细胞经过磁场处理后,Cx32的表达减少了,神经球和数量也有减少的趋势。结论:外加磁场影响缝隙连接的表达可能遵照霍尔效应原理。同样,中枢神经系统中,生物电磁场可能如同电磁泵样调节着缝隙连接通讯,以适应神经发育、细胞迁移、细胞的同步化等活动。这可能是早期神经干细胞以及成年时星形胶质等细胞缝隙连接工作的重要原理。
OBJECTIVE: Nerve stem cell gap junctional communication in the central nervous system plays an important role in neurogenesis and cell migration, while the gap junction as an electrical synapse is susceptible to electromagnetic mechanics. Experimental study of the magnetic field on rat embryonic spinal cord neural stem cells gap junctional role. METHODS: The experiment was performed at the Institute of Field Surgery, Third Military Medical University from January to October 2004. Application of 13E rat spinal cord neural stem cells cultured in vitro, while the magnetic field direction and the bottom of the dish perpendicular to the constant magnetic field treatment. The change of connexin32 (Cx32) was observed under laser scanning confocal microscope. Results: Under the microscope, the expression of Cx32 in NSCs without magnetic field treatment was very obvious. However, the expression of Cx32 was decreased and the numbers of neurospheres were also reduced after cultured with NSCs under the same conditions. Conclusion: The effect of applied magnetic field on the gap junction may follow the Hall effect principle. Similarly, in the central nervous system, the bioelectromagnetic field may act as a solenoid-pump-like regulator of gap junctional communication to accommodate neurodevelopment, cell migration, and cell synchronization. This may be an important principle for the early neural stem cells and the gap junction work of astrocytes and other adult cells.