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目的:建立硫酸阿托品的表面增强拉曼光谱(SERS)技术检测方法.方法:通过理论计算与试验检测相结合,对硫酸阿托品分子的拉曼峰进行归属,并选取1002 cm-1拉曼峰作为特征峰,开展定量分析.结果:硫酸阿托品水溶液中的检测限约为0.5μg×ml-1;1002 cm-1处特征峰强度随水溶液浓度变化关系的线性范围在1~8μg·ml-1,r=0.9839;平均回收率为103.3%,RSD为4.5%(n=9).同时,对该方法的批次间稳定性进行了测试,相对标准偏差为5.7%.另外,对含有硫酸阿托品的中药苍术掺杂样品进行检测,依然能检测到1002 cm-1处的特征峰.结论:该方法具有快速、准确、无损、操作简便等优点,在硫酸阿托品检测方面具有很好的应用潜力.“,”Objective:To establish a surface enhanced Raman spectroscopy ( SERS) technique for the determination of atropine sulfate. Methods:The Raman peaks of atropine sulfate molecules were classified by theoretical calculations and experimental tests, and the 1002 cm-1 Raman peaks were selected as the characteristic peaks for the quantitative analysis. Results:The detection limit of atropine sulfate in aqueous solution was below 0. 5 μg·ml-1 . The relationship between the intensity of characteristic peaks at 1002 cm-1 and the concentration of aqueous solution was linear within the range of 1-8 μg ml-1 with the linear correlation coefficient r of 0. 9839. The recovery rates of 2, 5 and 7μg ml-1 were measured, which were 97. 1%-109. 8%. The average recovery was 103. 3%, and the RSD was 4. 5% (n=9). At the same time, the stability of the method among the batches was tested, and the relative standard deviation was 5. 7 %. In addition, the atractylodes rhizome samples containing atropine sulfate were detected,and the characteristic peaks still could be detected at 1002 cm-1 . Conclusion:The method is rapid, accurate and nondestructive with easy operation, which can be used for the detection of atropine sulfate.