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目的建立一种用于定量检测鼠疫单克隆抗体样品中IgG含量的方法 ,以更好地指导杂交瘤细胞的筛选和抗体的生产、纯化。方法采用羊抗鼠IgG为包被抗体、辣根过氧化物酶标记的羊抗鼠IgG为标记抗体,纯化鼠IgG作参考品,制作标准曲线,测定未知样品中的IgG含量。结果该方法与BCA蛋白浓度测定法测得的结果基本一致,且重复性好,特异性强,灵敏度高。结论双抗体夹心ELISA法可用于测定鼠疫F1单克隆抗体样品中的IgG含量。
OBJECTIVE: To establish a method for the quantitative determination of IgG in samples of monoclonal antibodies against plague, in order to better guide the screening of hybridoma cells and the production and purification of antibodies. Methods Goat anti-mouse IgG was used as coated antibody, horseradish peroxidase-labeled goat anti-mouse IgG as labeled antibody and purified mouse IgG as reference material to prepare a standard curve for the determination of IgG in unknown samples. Results The method was consistent with the results of BCA protein concentration determination, and the repeatability, specificity and sensitivity were high. Conclusion The double antibody sandwich ELISA method can be used to determine the IgG content of the plague F1 monoclonal antibody sample.