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目的:目前周围神经修复中,神经导管是研究热点,本文研究乳液法涂层纤维制备的神经导管在神经修复中应用的可能性。方法:本文采用乳液法制备担载NGF的丝素-聚乳酸(PLLA)涂层电纺纤维,观察纤维的形态,测定NGF的体外释放动力学参数,并考察纤维释放液对于PC12细胞增殖的影响。结果:担载NGF的涂层纤维具备类似于细胞外基质(ECM)的三维结构和多孔形态;涂层纤维中NGF体外有效缓释10天;细胞实验中,在含有释放液的培养基中生长的PC12细胞,与空白对照组相比,荧光强度平均多了2000-4000个荧光强度,所以释放液可以更好地促进PC12细胞的增殖。结论:担载NGF的乳液法涂层纺丝纤维具备促进缺损周围神经修复的条件,可以进一步研究在动物体内修复缺损周围神经中的效果,为以后的临床应用打下基础。
OBJECTIVE: In the current peripheral nerve repair, the nerve catheter is a hot research topic. This article studies the possibility of the application of the nerve catheter prepared by emulsion fiber coating in nerve repair. Methods: Fibroin-polylactic acid (PLLA) coated electrospun fibers coated with NGF were prepared by emulsion method. The morphologies of the fibroin-PLLA coated nanofibers were observed. The kinetic parameters of NGF release in vitro were measured. The effects of fiber release on the proliferation of PC12 cells . Results: The coated fibers with NGF possessed the three-dimensional structure and porous morphology similar to that of extracellular matrix (ECM). NGF in coated fibers was effectively released in vitro for 10 days in vitro. In cell experiments, Of PC12 cells, compared with the blank control group, the average fluorescence intensity of 2000-4000 fluorescence intensity, so the release of liquid can better promote PC12 cell proliferation. CONCLUSION: The emulsion-coated, coated spinning fibers loaded with NGF have the conditions to promote peripheral nerve repair of defects, and further study the effect of repairing the peripheral nerve defect in animals, which will lay the foundation for future clinical application.