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采用两步蒸馏沉淀聚合法,制备了红霉素表面分子印迹聚合物.第一步制备了甲基丙烯酸和二乙烯基苯共聚物微球(PMAA-co-DVB),将其作为内核,再通过第二步蒸馏沉淀聚合,以甲基丙烯酸(MAA)为功能单体,二乙烯基苯(DVB)为交联剂,偶氮二异丁腈(AIBN)为引发剂,红霉素(EM)为模板分子,内核表面聚合一层分子印迹膜层,从而制得具有“核-壳”结构的红霉素表面分子印迹聚合物.通过激光粒径测定、扫描电子显微镜以及结合实验对其形貌和结合性能进行表征.结果表明,在聚合物单体体积分数为7%时,制备出的PMAA-co-DVB微球形态良好,当EM与MAA的比例为1∶4,第二步蒸馏沉淀聚合中单体体积分数为2.8%时,所得的印迹材料结合性能最佳,对EM最大结合量为76.8 mg/g,结合达到平衡的时间为90 min.对红霉素的识别能力高于对其结构类似物罗红霉素.
A two-step distillation and precipitation polymerization method was used to prepare erythromycin surface molecularly imprinted polymers. The first step was to prepare the microspheres of methacrylic acid and divinylbenzene copolymer (PMAA-co-DVB) Through the second step of precipitation polymerization, methacrylic acid (MAA) as the functional monomer, divinylbenzene (DVB) as the crosslinking agent, azobisisobutyronitrile (AIBN) as the initiator, erythromycin ) As the template molecule, a layer of molecularly imprinted membrane was polymerized on the surface of the core to prepare an erythromycin surface molecularly imprinted polymer with “core-shell” structure. By laser particle size determination, scanning electron microscopy and binding experiments Its morphology and binding properties were characterized.The results showed that PMAA-co-DVB microspheres prepared in good condition when the volume fraction of polymer monomer was 7%, when the ratio of EM to MAA was 1: 4, the second When the volume fraction of monomer in step distillation polymerization is 2.8%, the best binding property of the imprinted material is obtained, the maximum binding capacity to EM is 76.8 mg / g and the binding time reaches 90 min. Roxithromycin above its structural analogues.