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目的研究柚皮苷对配体门控离子通道7嘌呤能P2X受体(P2X7)介导的1型人类免疫缺陷病毒(HIV-1)包膜糖蛋白120(gp120)所致大鼠认知障碍的保护作用。方法水迷宫实验观察柚皮苷对侧脑室灌注gp120拟痴呆大鼠认知功能障碍的影响,反转录PCR检测海马组织P2X7受体mRNA的水平,Western blot法检测海马组织P2X7受体蛋白的水平。结果 Morris水迷宫可见柚皮苷治疗组大鼠的逃避潜伏期和寻找目标错误次数与gp120模型组相比缩短。Western blot法和PCR检测结果显示,柚皮苷治疗组P2X7受体蛋白和mRNA的表达与模型组相比有所下降。结论柚皮苷具有改善侧脑室灌注gp120所致大鼠学习记忆障碍的作用,其机制可能与对抗P2X7受体表达上调有关。
Objective To study the effect of naringin on cognitive impairment in rats induced by ligand-gated ion channel 7 purinergic P2X receptor (P2X7) mediated by human immunodeficiency virus type 1 (HIV-1) glycoprotein 120 (gp120) The protective effect. Methods The water maze test was used to observe the effect of naringin on the cognitive dysfunction induced by lateral ventricle perfusion of gp120 in dementia rats. The level of P2X7 receptor mRNA in hippocampus was detected by reverse transcription polymerase chain reaction (RT-PCR). The level of P2X7 receptor protein . Results In the Morris water maze, the escape latency and the number of search errors in the naringin-treated group were shorter than those in the gp120 model group. Western blot and PCR results showed that the expression of P2X7 receptor protein and mRNA in naringin group decreased compared with model group. Conclusion Naringin can improve learning and memory impairment induced by intracerebral perfusion of gp120 in rats. The mechanism may be related to up-regulation of P2X7 receptor expression.