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Bambusa pervariabilis × Dendrocalamopsis grandis blight is caused by a toxin from the fungus Arthrinium phaeospermum(corda) M. B. Ellis. We used shaking culture in a modified Fries culture medium and methanol extraction to isolate the toxin. The optimal developing solvent mixture(methanol: ethyl acetate: H2O at 7:1.5:3) was selected using thin layer chromatography and used as the eluent for toxin purification by silica gel column chromatography. Two toxic fractions were identified in the bioassay. A flaxen oil substance, AP-Ⅰ, showed higher toxicity than a toxic white powder, AP-Ⅱ. The more toxic AP-Ⅰ was determined to be dibutyl phthalate(C16H22O4, molecular weight of 278) by mass spectrometry, nuclear magnetic resonance, and infrared spectrophotometry. Dibutyl phthalate might contribute to the pathogenesis of bamboo blight.
Bambusa pervariabilis × Dendrocalamopsis grandis blight is caused by a toxin from the fungus Arthrinium phaeospermum (corda) MB Ellis. We used shaking culture in a modified Fries culture medium and methanol extract to isolate the toxin. The optimal developing solvent mixture (methanol: ethyl acetate Was selected using thin layer chromatography and used as the eluent for toxin purification by silica gel column chromatography. Two toxic fractions were identified in the bioassay. A flaxen oil substance, AP-I, showed higher toxicity than a toxic white powder, AP-II. The more toxic AP-I was determined to be dibutyl phthalate (C16H22O4, molecular weight of 278) by mass spectrometry, nuclear magnetic resonance, and infrared spectrophotometry. Dibutyl phthalate might contribute to the pathogenesis of bamboo blight.