论文部分内容阅读
Melanoma is the most malignant and aggressive form of skin carcinoma originating in the pigment-producing melanocytes.In this study,to further investigate the molecular mechanisms of the development and progression of melanoma,we explored the impacts of long non-coding RNA (lncRNA) CASC2 on melanoma cell functions.Microarray analysis was carried out to identify the expression of lncRNA CASC2 in melanoma cells.MiR-181a was predicted as a sponging target of CASC2 by miRcode,while the 3’-UTR of Plexin C1 (PLXNC1) was a potential target of miR-181a according to the TargetScan database.The correlation among CASC2,miR-181a,and PLXNC1 was verified by dual luciferase reporter assay and qRT-PCR.After manipulation of CASC2,miR-181a and PLXNC1 expression with transfection in A375 and M14 cells,cell viability,apoptosis,and invasive ability were evaluated using CCK-8,flow cytometry and Transwell assays,respectively.A low expression of CASC2 was detected in melanoma tissues and cells.Dual luciferase reporting assay confirmed that miR-181a targeted the 3’-UTR of PLXNC1.Furthermore,CASC2 could efficiently sponge miR-181a,thereby facilitating the expression of PLXNC1.Up-regulation of CASC2 suppressed the cell proliferation and invasion,but induced the apoptosis of melanoma cells.Our results demonstrated that lncRNA CASC2 can promote PLXNC1 expression by sponging miR-181a,thereby inhibiting the proliferation and invasion of melanoma cells,indicating that lncRNA CASC2 functions via the miR-181a/PLXNC1 axis in melanoma.