论文部分内容阅读
目的:建立黄连上清胶囊中栀子苷的高效液相含量测定方法。方法:采用高效液相色谱法(HPLC),色谱柱为BOSTON C_(18)(250 mm×4.6,5μm),流动相为乙腈-水(15∶85),流速为1.0 m L/min,检测波长为238 nm。结果:栀子苷浓度在0.041 2~0.823 0μg/m L范围内呈良好的线性关系(r=0.999 9),平均回收率为99.31%,RSD为0.72%(n=6),3批样品的平均含量为1.5842μg/g。结论:该方法简便、快速、重现性好,可作为黄连上清胶囊的质量控制。
Objective: To establish a HPLC method for the determination of geniposide in Huanglian Shangqing Capsule. METHODS: The mobile phase consisted of acetonitrile-water (15:85) with a flow rate of 1.0 mL / min using high performance liquid chromatography (HPLC) with BOSTON C 18 (250 mm × 4.6 and 5 μm) The wavelength is 238 nm. Results: The calibration curve of geniposide showed a good linearity (r = 0.999 9) in the range of 0.041 2 ~ 0.823 0 μg / m L with an average recovery of 99.31% and a RSD of 0.72% (n = 6) The average content of 1.5842μg / g. Conclusion: The method is simple, rapid, reproducible, and can be used as quality control of Huanglian Shangqing Capsule.