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为探讨胃恶性淋巴瘤的发病机制和实验治疗提供理想的动物模型。将 11例胃恶性淋巴瘤新鲜组织移植裸鼠胃粘膜下层 ,观察原位移植成瘤率 ,移植瘤的侵袭和转移 ,及其形态学特征 (光镜 ,电镜 ,免疫组化)。从 11例胃恶性淋巴瘤标本中筛选出 1株人胃恶性淋巴瘤裸鼠原位移植高转移模型和 1株人胃恶性淋巴瘤裸鼠原位移植模型 ,分别传至 36代和 2 7代 ,共移植裸鼠 32 9只。自第 3代起移植成瘤率及液氮冻存复苏成活率均为 10 0 %。出现淋巴道、血道及种植转移。原位移植瘤的病理组织学、超微结构、DNA含量测定及染色体核型分析结果均与来源人胃恶性淋巴瘤相似。人胃恶性淋巴瘤在裸鼠胃内自主生长 ,浸润破坏胃壁各层组织结构。表明此模型可用于胃恶性淋巴瘤的发病机制、侵袭、转移及实验治疗的研究
In order to explore the pathogenesis of gastric malignant lymphoma and experimental treatment to provide the ideal animal model. 11 cases of gastric malignant lymphoma were transplanted into the submucosa of fresh gastric mucosa in nude mice. The tumorigenic rate and the invasion and metastasis of the transplanted tumor were observed. The morphological features (light microscope, electron microscope and immunohistochemistry) were observed. One human gastric malignant lymphoma in situ transplanted high metastasis model and one human gastric malignant lymphoma orthotopic transplantation model were screened from 11 gastric malignant lymphoma specimens and were respectively transmitted to 36 and 27 generation , A total of 329 nude mice were transplanted. Since the third generation, the rate of tumorigenesis and the survival rate of liquid nitrogen cryopreservation were 100%. Appear lymphatic, blood and plant transfer. Histopathology, ultrastructure, DNA content and chromosomal karyotype analysis of orthotopic xenografts were similar to those of human gastric malignant lymphoma. Human gastric malignant lymphomas grow independently in the stomach of nude mice and infiltrate and destroy the tissue structure of the stomach wall. This model can be used to study the pathogenesis, invasion, metastasis and experimental treatment of gastric malignant lymphoma