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目的:建立RP-HPLC法同时测定冲和软膏中芍药苷和蛇床子素含量的方法。方法:色谱柱为Phenomisl C18柱(150 mm×4.6 mm5,μm);以乙腈-水为流动相进行梯度洗脱;流速为1.0 mL·min-1;柱温为35℃;检测波长为230和330 nm。结果:芍药苷与蛇床子素的线性范围分别为3.12～249.8μg·mL-1(r=0.999 8)和1.54～123μg·mL-1(r=0.999 9);平均加样回收率分别为101.0%(RSD=1.07%,n=9)和100.8%(RSD=1.21%,n=9)。结论:本方法操作简便,测定结果准确,重复性好,可作为该产品质量控制的方法。
Objective: To establish a method for the simultaneous determination of paeoniflorin and osthole in Chonghe Ointment by RP-HPLC. METHODS: The column was Phenomisl C18 column (150 mm × 4.6 mm 5, μm). The mobile phase consisted of acetonitrile and water. The flow rate was 1.0 mL · min -1 and the column temperature was 35 ℃. The detection wavelength was 230 and 330 nm. Results: The linear ranges of paeoniflorin and osthole were 3.12 ~ 249.8 μg · mL -1 (r = 0.999 8) and 1.54 ~ 123 μg · mL -1 (r = 0.999 9), respectively. The average recoveries of paeoniflorin and osthole were 101.0 % (RSD = 1.07%, n = 9) and 100.8% (RSD = 1.21%, n = 9). Conclusion: The method is simple, accurate and reproducible, and can be used as a method of product quality control.