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目的探讨胰腺星状细胞(PSCs)对胰腺癌细胞PANC-1迁移能力的影响。方法从小鼠胰腺组织中分离PSCs后分为A组(共培养PSCs及PANC-1细胞)和B组(PANC-1细胞),均以Transwell小室培养24h。细胞划痕及细胞迁移实验分别检测两组PANC-1细胞的迁徙距离和跨膜细胞数目,免疫荧光技术及Western blot测定两组PANC-1细胞中基质金属蛋白酶(MMP)-2和MMP-9的表达。结果培养24h后,A组细胞迁移距离远于B组[(18.75±1.70)mm vs.(12.40±1.14)mm](P<0.05),跨膜细胞数目多于B组[(137±24)个vs.(68±11)个](P<0.05),MMP-2和MMP-9蛋白表达量均高于B组(0.84±0.08vs.0.41±0.13和0.56±0.72vs.0.31±0.12)(P<0.05)。结论 PSCs能够增强PANC-1细胞迁移能力,促进PANC-1细胞中MMP-2、MMP-9的表达。
Objective To investigate the effect of pancreatic stellate cells (PSCs) on the migration of pancreatic cancer cell PANC-1. Methods PSCs were isolated from mouse pancreatic tissues and divided into group A (co-cultured PSCs and PANC-1 cells) and group B (PANC-1 cells), all cultured in Transwell chamber for 24h. The migration distance and the number of transmembrane cells of PANC-1 cells were detected by cell scratch assay and cell migration assay respectively. The expressions of MMP-2 and MMP-9 in PANC-1 cells were detected by immunofluorescence and Western blot expression. Results The number of transmembrane cells in group A was significantly longer than that in group B [(18.75 ± 1.70) mm vs. (12.40 ± 1.14) mm] (P < (68 ± 11)] (P <0.05). The protein expressions of MMP-2 and MMP-9 in group B were significantly higher than those in group B (0.84 ± 0.08 vs.0.41 ± 0.13 and 0.56 ± 0.72 vs.0.31 ± 0.12) (P <0.05). Conclusion PSCs can enhance the migration of PANC-1 cells and promote the expression of MMP-2 and MMP-9 in PANC-1 cells.