Analysis of SSRs Derived from an EST Library of Half-Smooth Tongue Sole Cynoglossus semilaevis

• 作 者: LIU Yunguo BAO Baolong CHEN So
• 机 构: CollegeofFisheries,KeyLaboratoryofMarineandEstuarineFisheries,CollegeofAqua-Life,ScienceandTechnolog
• 出 处: 中国海洋大学学报：英文版
• 发表日期: 2008年3期
• 关键词: 半滑舌鳎 分析方法 基因 鱼 half-smooth tongue sole Cynoglossus semilaevis EST microsatell
• 基金项目: AcknowledgementsThis work was supported by grants from the Key Laboratory of Marine and Estuarine Fisheries, the Ministry of Agriculture （05-03-01）, the Key Laboratory of Aquatic Genetic Resources and

A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(EST) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequenced. A total of 1060 non-redundant simpl