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目的探讨雌激素硫酸基转移酶(SULT1E1)和孕激素受体蛋白(PR)在子宫肉瘤组织中的表达并分析其与子宫肉瘤发病的相关性。方法选取2001—2010年中国医科大学附属盛京医院妇产科收治的子宫肉瘤病人石蜡病理切片46例(子宫内膜间质肉瘤31例,子宫平滑肌肉瘤15例),取宫颈上皮内瘤变(CIN)Ⅲ级或子宫脱垂病人的正常子宫肌层组织石蜡病理切片20例作为对照。用免疫组化法对子宫肉瘤组织的SULT1E1、PR蛋白进行检测。结果子宫内膜间质肉瘤组及子宫平滑肌肉瘤组分别与正常子宫肌层组SULT1E1蛋白阳性率比较,差异均有统计学意义(均P<0.05);子宫内膜间质肉瘤组与子宫平滑肌肉瘤组PRA蛋白、PRB蛋白阳性率比较差异均有统计学意义(均P<0.05)。子宫内膜间质肉瘤组织中SULT1E1与PRA、PRB蛋白之间均呈负相关关系(分别r=-0.389,P=0.031;r=-0.421,P=0.018)。结论子宫肉瘤的发生、发展与子宫肉瘤组织SULT1E1蛋白表达降低或缺失有关;在子宫内膜间质肉瘤组织中SULT1E1的表达与孕激素受体A、B表达均呈负相关。
Objective To investigate the expression of estrogen sulfotransferase (SULT1E1) and progesterone receptor protein (PR) in uterine sarcoma and its relationship with the incidence of uterine sarcoma. Methods From 2001 to 2010, 46 patients with paraffin sections of uterine sarcoma (31 cases of endometrial stromal sarcoma and 15 cases of uterine leiomyosarcoma) were enrolled in Department of Obstetrics and Gynecology of Shengjing Hospital affiliated to China Medical University. Cervical intraepithelial neoplasia CIN) Ⅲ grade or uterine prolapse in patients with normal myometrium paraffin pathological section of 20 cases as a control. Immunohistochemistry was used to detect SULT1E1 and PR in uterine sarcoma. Results The positive rates of SULT1E1 protein in endometrial stromal sarcoma group and uterine leiomyosarcoma group were significantly different from those in normal myometrium group (all P <0.05). The positive rates of SULT1E1 protein in endometrial stromal sarcoma group and uterine leiomyosarcoma group were significantly higher than those in normal uterine leiomyosarcoma group The positive rates of PRA protein and PRB protein in the two groups were significantly different (all P <0.05). There was a negative correlation between SULT1E1, PRA and PRB in endometrial stromal sarcoma (r = -0.389, P = 0.031; r = -0.421, P = 0.018, respectively). Conclusions The occurrence and development of uterine sarcoma are related to the decrease or deletion of SULT1E1 protein in uterine sarcoma. The expression of SULT1E1 in endometrial stromal sarcoma is negatively correlated with the expression of progesterone receptor A and B.