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目的 研究外源p73基因转染wtp5 3型人肺腺癌A5 49细胞对其化疗药物敏感性的影响。 方法 用脂质体介导的转染技术 ,将含有全长人野生型 p73αcDNA和野生型 p5 3cDNA的真核表达重组质粒分别导入A5 49细胞 ,观察基因转染前后肿瘤细胞对化疗药物顺铂和阿霉素敏感性的变化。结果 A5 49 p73α细胞可以稳定地表达P73α蛋白 ,其生长速度较未转染p73α和转染wtp5 3基因的A5 49细胞明显减慢 ,克隆形成数下降 ,凋亡细胞明显增多。与未转染组比较 ,在原本没有抑制和杀伤作用的化疗药物浓度作用下A5 49 p73α细胞生长明显受到抑制 ,顺铂和阿霉素的IC50 值分别降低为约 1/6和 1/70。结论 研究显示外源性 p73基因的导入增加了wtp5 3型人肺腺癌A5 49细胞对顺铂和阿霉素等化疗药物的敏感性 ,为 p73基因用于治疗wtp5 3不能发挥作用的恶性肿瘤提供实验依据
Objective To investigate the effect of exogenous p73 gene transfected wtp5 3 human lung adenocarcinoma A549 cells on chemosensitivity. Methods The eukaryotic recombinant plasmids containing full-length human wild-type p73α cDNA and wild-type p5 3 cDNA were transfected into A549 cells respectively by liposome-mediated transfection. The effect of tumor cells on cisplatin and Changes in doxorubicin sensitivity. Results A549 p73α cells stably expressed P73α protein. The growth rate of A549 p73α cells was significantly slower than that of untransfected p73α and wtp5 3 transfected A549 cells. The number of cloned cells decreased and the number of apoptotic cells increased significantly. Compared with untransfected A549 cells, the growth of A549 p73α cells was significantly inhibited by the concentration of chemotherapeutic drugs that did not inhibit or kill A549 cells. The IC50 values of cisplatin and doxorubicin were reduced to about 1/6 and 1/70, respectively. Conclusions The study showed that the introduction of exogenous p73 gene increased the sensitivity of wtp5 3 human lung adenocarcinoma A549 cells to chemotherapeutic drugs such as cisplatin and doxorubicin, and that the p73 gene could be used to treat malignant tumors in which wtp5 3 did not play a role Provide experimental basis