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目的:探讨氧化苦参碱(OMT)对大鼠缺血再灌注心肌损伤(MIRI)的保护机制。方法:随机将60只成年Wistar大鼠分成对照组、MIRI组和OMT组,每组20只,除对照组外,其他两组结扎30 min后松解结扎线灌注60 min。结扎前10 min,OMT组股静脉输入苦参注射液120 mg/kg,对照组、MIRI组则输入等容量生理盐水。造模后,记录两组心率(HR)、左心室收缩压(LVSP)、左室内压最大上升或下降速率(+dp/dt_(max)或-dp/dt_(min))及血清乳酸脱氢酶(LDH),检测两组心肌组织中一氧化氮(NO)、丙二醛(MDA)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)水平。结果:与MIRI组相比,OMT组HR、LVSP和+dp/dt_(max)、-dp/dt_(min)均显著升高(P<0.05),且OMT组上述指标与对照组比较,差异均无统计学意义(均P>0.05)。与MIRI组相比,OMT组的NO、NOS、SOD、GSH-PX水平均显著升高,而MDA、血清LDH水平显著降低,比较差异均有统计学意义(均P<0.05),且OMT组上述指标与对照组比较,差异均无统计学意义(均P>0.05)。结论:OMT对MIRI大鼠具有心肌保护作用,其机制可能与提高抗氧自由基活性、改善微循环及舒张冠脉血管有关。
Objective: To investigate the protective mechanism of oxymatrine (OMT) on myocardial ischemia-reperfusion injury (MIRI) in rats. Methods: 60 adult Wistar rats were randomly divided into control group, MIRI group and OMT group, with 20 rats in each group. Except the control group, the other two groups were ligated for 30 minutes after ligation for 60 minutes. 10 minutes before ligation, the OMT group femoral vein injection of matrine 120 mg / kg, the control group, MIRI group, the same volume of saline input. After modeling, the heart rate (HR), left ventricular systolic pressure (LVSP), maximum increase or decrease of left ventricular pressure (+ dp / dt max or -dp / dt min) and serum lactate dehydrogenation Enzyme linked immunosorbent assay (LDH) was used to detect the levels of NO, MDA, NOS, SOD and GSH in the two groups Enzyme (GSH-PX) levels. Results: Compared with MIRI group, HR, LVSP, + dp / dt max and dp / dt min increased significantly in OMT group (P <0.05), and OMT group compared with the control group No statistical significance (all P> 0.05). Compared with the MIRI group, the levels of NO, NOS, SOD and GSH-PX in OMT group were significantly increased, while the levels of MDA and serum LDH in OMT group were significantly decreased (all P <0.05) The above indicators compared with the control group, the difference was not statistically significant (P> 0.05). CONCLUSION: OMT can protect myocardium of MIRI rats. Its mechanism may be related to improving the activity of antioxidant free radicals, improving microcirculation and relaxing coronary vessels.