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目的检测儿童急性淋巴细胞白血病细胞增殖性抗原表达情况，观察其与预后的关系。方法用流式细胞仪结合细胞增殖性抗原ＣＤ７１、Ｋｉ６７和增殖细胞核抗原（ＰＣＮＡ）检测１２名正常儿童和３５例急性淋巴细胞白血病（ＡＬＬ）患儿骨髓细胞的表达率，观察治疗前后增殖指数（ＬＩ）的变化及其与预后的关系。结果正常儿童ＣＤ７１ＬＩ为（３２．１８±１６．６６）％，ＡＬＬ患儿为（３３．６６±２１．５２）％，两组相比，差异无显著性（ｔ＝０．０５１２，Ｐ＞０．０５）；正常儿童Ｋｉ６７ＬＩ和ＰＣＮＡＬＩ分别为（４．８２±９．２７）％和（１９．９６±２５．１１）％，ＡＬＬ患儿分别为（３２．１４±２３．５９）％和（４７．４６±３０．９６）％，两组比较，差异极为显著（ｔ＝３．２０６，Ｐ＜０．０１和ｔ＝２．８７９，Ｐ＜０．０１）。初诊时白血病细胞的ＰＣＮＡＬＩ为（４７．４６±３０．９６）％，经治疗缓解３年时为（２７．２８±１２．５１）％，已趋于正常。３５例中２８例持续缓解的患儿初诊时ＰＣＮＡＬＩ为（５２．５９±３２．００）％，而预后差的７例患儿初诊时为（２６．９４±１４．４８）％，两者比较，差异有显著性（Ｆ＝８．８７７，Ｐ＜０．０１）。结论白血病?
Objective To detect the expression of proliferating antigen in children with acute lymphoblastic leukemia and to observe its relationship with prognosis. Methods The expression of bone marrow cells in 12 normal children and 35 children with acute lymphoblastic leukemia (ALL) were detected by flow cytometry combined with cell proliferating antigen CD71, Ki67 and proliferating cell nuclear antigen (PCNA). Proliferation Change of index (LI) and its relationship with prognosis. Results The positive rate of CD71LI was (32.18 ± 16.66)% in normal children and (33.66 ± 21.52)% in ALL children. There was no significant difference between the two groups (t = 0.0512, P> 0 .05). The normal children Ki-67LI and PCNALI were (4.82 ± 9.27)% and (19.96 ± 25.11)%, respectively. The children with ALL were (32.14 ± 23.59)% And (47.46 ± 30.96)% respectively. There was significant difference between the two groups (t = 3.206, P <0.01 and t = 2.879, P <0.01). The PCNALI of leukemia cells was (47.46 ± 30.96)% at initial diagnosis, and (27.28 ± 12.51)% at 3 years after treatment, which tended to be normal. Among the 35 cases, 28 patients with sustained remission had a PCNALI of (52.59 ± 32.00)% at the time of initial diagnosis, and 7 patients with poor prognosis were (26.94 ± 14.48)% at the time of initial diagnosis , The difference was significant (F = 8.877, P <0.01). Conclusion Leukemia?