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目的:研究三氧化二砷(As2O3)诱导子宫颈癌细胞系HeLa细胞凋亡,探讨As2O3 对子宫颈癌的临床应用意义。方法:采用四甲基偶氮唑盐比色(MTT)法检测浓度分别为1.0、2.0、5.0、10.0μmol/L的As2O3 作用1、2、3、4 d后HeLa细胞的存活率,并进行形态学观察;采用细胞凋亡原位检测(TUNEL)法进行细胞凋亡的检测。结果:2.0μmol/L As2O3 处理HeLa细胞48 h后,可见细胞的存活率明显降低(P<0.05),处理96 h后,细胞的存活率进一步降低(P<0.01),经5.0μmol/L As2O3 处理的HeLa细胞24 h后就明显可见细胞的存活率降低;As2O3 作用后,HeLa细胞具有明显的凋亡特征性改变;TUNEL法检测可发现HeLa细胞有DNA的断裂。结论:As2O3 可以诱导子宫颈癌HeLa细胞凋亡,抑制细胞增殖,随着As2O3 浓度增加,作用时间延长,效果越明显,其作用具有浓度和时间依赖性。
Objective: To study the apoptosis of cervical cancer cell line HeLa induced by As2O3 and explore the clinical significance of As2O3 in cervical cancer. Methods: The viability of HeLa cells treated with 1.0,2.0,5.0 and 10.0μmol / L As2O3 for 1, 2, 3 and 4 days were detected by MTT assay Morphological observation was performed. Cell apoptosis was detected by TUNEL method. RESULTS: After treated with 2.0μmol / L As2O3 for 48 h, the survival rate of HeLa cells was significantly decreased (P <0.05). After 96 h treatment, the survival rate of HeLa cells was further decreased (P <0.01). After treated with 5.0μmol / L As2O3 HeLa cells treated with HeLa significantly decreased the viability of HeLa cells after 24 h treatment. HeLa cells showed obvious apoptotic changes after treated with As2O3. TUNEL assay showed that HeLa cells had DNA breaks. Conclusion: As2O3 can induce apoptosis of cervical cancer HeLa cells and inhibit cell proliferation. With the increase of As2O3 concentration, the effect is prolonged and the effect is more obvious. The effect of As2O3 is concentration-dependent and time-dependent.