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为了从遗传基础广泛的白菜型油菜资源中筛选出抗旱材料,并进一步进行抗旱相关基因筛选提供材料。用不同浓度PEG溶液于萌发期对收集的45份油菜种质资源进行处理,观察并测定油菜种子萌发数、根长、芽长,计算发芽率、发芽势、相对发芽率、相对发芽势,与对照相比,PEG胁迫下发芽率有显著差异,对各指标进行主成分分析,确定抗旱性鉴定参数,用综合隶属函数平均值进行抗旱性筛选,并对所有供试材料抗旱性进行排序,鉴定出抗旱性较强的白菜型油菜,然后对筛选出的材料在苗期进行PEG干旱胁迫,验证其抗旱性。模拟干旱胁迫下,鉴定出一份抗旱性强的白菜型油菜,其来源为门源浩门蜡烛油菜。用PEG模拟干旱胁迫进行白菜型油菜抗旱种质资源筛选方法可行。通过培养皿和花盆苗期PEG诱导鉴定出的抗旱种质资源可进一步进行抗旱相关基因筛选。
In order to select drought-resistant materials from a wide range of genetically-selected Brassica campestris resources, and further screening of drought-related genes to provide materials. The germplasm resources of 45 rapeseed collected at the germination stage were treated with different concentrations of PEG solution. The number of germination, root length and bud length, the germination rate, germination potential, relative germination rate, relative germination potential, Compared with the control, the germination rate was significantly different under PEG stress. Principal component analysis (PCA) of each index was carried out to determine the identification parameters of drought resistance, and the drought resistance was screened by the average of the membership functions. The drought resistance of all the tested materials was ranked and identified A more drought-resistant cabbage rapeseed, and then screened material PEG drought stress at seedling stage, to verify the drought resistance. Simulated drought stress, identified a drought-resistant Brassica campestris, the source of the door source Hao door candle rape. Drought-resistant Germplasm Resources Screening of Brassica campestris L. Using PEG Simulated Drought Stress is Possible. Drought-related germplasm resources can be further screened through drought-resistant germplasm resources induced by PEG in petri dishes and flower pots.