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目的 目前慢性粒细胞性白血病 (CML)的化疗效果仍不理想 ,为寻找对CML敏感的药物 ,该研究探讨四硫化四砷、STI5 71和除莠霉素对CML细胞株K5 6 2的作用。方法 通过MTS方法检测三种药物在不同浓度 (0 .0 1 ,0 .1 ,1 .0 ,2 .0 ,5 .0 ,1 0 .0 μmol/L)下对K5 6 2细胞活力的影响。采用瑞氏染色、荧光染色和琼脂糖凝胶电泳观察药物处理后细胞形态学的变化和细胞凋亡的发生。用流式细胞仪检测细胞凋亡率。结果 用 5 .0 μmol/L的四硫化四砷、STI5 71和除莠霉素培养 3天 ,K5 6 2细胞活力明显下降 ,其吸光值分别为 0 .32± 0 .0 4 ,0 .4 9± 0 .0 1 ,0 .6 9± 0 .0 2 ,其中四硫化四砷和STI5 71对细胞的抑制作用强于除莠霉素 (P <0 .0 1 ) ,前两者间无明显差异 (P >0 .0 5 )。 1 .0 ,5 .0 ,1 0 .0 μmol/L的四硫化四砷对K5 6 2细胞的抑制作用呈时间依赖关系 (P <0 .0 1 )。小于 1 .0μmol/L的四硫化四砷对K5 6 2细胞活力无明显影响。四硫化四砷培养 2 4至 4 8小时后 ,K5 6 2细胞出现染色质浓缩、核碎片及凋亡小体等典型的凋亡形态学改变。用 5 .0 μmol/L的四硫化四砷、STI5 71和除莠霉素培养 72小时后 ,K5 6 2细胞的凋亡率分别为 6 8.8%、5 6 .7%和 35 .5 % ,2 .0与 3.0 μmol/L的四硫化四砷诱导K5 6 2细胞凋?
Objective To investigate the effect of arsenic tetrasulfide, STI5 71 and herbimycin on the CML cell line K5 6 2 in order to find a chemotherapeutic effect of chronic myelogenous leukemia (CML). Methods The effects of three drugs on the viability of K562 cells at different concentrations (0.01, 0.1, 1.0, 2.0, 5.0 and 1.0 μmol / L) were determined by MTS method . Wright’s staining, fluorescence staining and agarose gel electrophoresis were used to observe the change of cell morphology and the occurrence of apoptosis after drug treatment. Flow cytometry was used to detect apoptosis rate. Results The viability of K5 6 2 cells was significantly decreased with 5.0 μmol / L arsenic tetrasulphide, STI5 71 and herbimycin for 3 days. The absorbance values of K5 6 2 cells were 0 .32 ± 0 .0 4 and 0.4 9 ± 0. 01 and 0. 69 ± 0. 0 2, respectively. The inhibitory effect of arsenic tetrasulfide and STI5 71 on the cells was stronger than that of herbimycin (P <0.01) Significant difference (P> 0.05). The inhibitory effect of arsenic tetrasulfide (Ⅳ) on K5 6 2 cells in a time-dependent manner at a concentration of 1.0,0.5 and 1.0 μmol / L was in a time-dependent manner (P <0.01). Arsenic tetrasulfide less than 1.0 μmol / L had no significant effect on the viability of K5 6 2 cells. K462 cells cultured in arsenic tetra-arsenide for 24 to 48 hours exhibited typical changes in apoptosis such as chromatin condensation, nuclear debris and apoptotic bodies. The apoptosis rate of K562 cells after treated with 5 .0 μmol / L arsenic tetrathionate, STI5 71 and herbimycin for 72 hours were respectively 6 8.8%, 56.7% and 35.5% 2 .0 and 3.0 μmol / L of arsenic tetrasulfide induced K562 cells wither?