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原位分子杂交方法具有敏感性高、定位准确、对抗原可从基因水平进行定位等优点,尤其对细胞外基质包括胶原蛋白的研究更为适合。因为这类基因的蛋白产物半衰期较长(数月至数年),免疫组化方法所检测的抗原蛋白无法区分陈旧蛋白和新合成的蛋白,而细胞外基质的mRNA则因其半衰期极短,只有数小时,且不能透过细胞膜而弥散,故其mRNA水平更能代表它们的合成状态。国外1988和1989年
In situ molecular hybridization has the advantages of high sensitivity, accurate positioning and positioning of antigens from the gene level, especially for extracellular matrix including collagen. Because of the long half-life (several months to several years) of the protein products of these genes, antigenic proteins detected by immunohistochemistry can not distinguish between obsolete proteins and newly synthesized proteins, whereas extracellular matrix mRNAs, due to their extremely short half-lives, Only a few hours, and can not be dispersed through the cell membrane, so its mRNA level more representative of their synthetic state. Foreign 1988 and 1989