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以60个国内外紫花苜蓿品种为试材,通过表型鉴定的方法筛选出耐盐、敏盐品种,并利用主成分分析和隶属函数法对初步筛选出品种的叶绿素含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性及丙二醛(MDA)含量进行综合评价,根据综合评价值(D值)对耐盐性进行强弱排序,以期为紫花苜蓿遗传群体的构建及耐盐性相关基因的发掘奠定基础。结果表明:在150mmol·L~(-1) NaCl胁迫下,‘Asi’>“兴平”>“秘鲁”>“草原3号”>国产苜蓿;在300 mmol·L~(-1) NaCl胁迫下,“阿迪娜”>“巨能”>“巨能7号”>“抗旱15”>“骑士T”。筛选出了耐盐、敏盐的种质资源材料。
Sixty alfalfa cultivars at home and abroad were used as materials to screen salt-tolerant and salt-sensitive varieties by phenotypic identification method. Principal component analysis and membership function were used to analyze the chlorophyll content, the content of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) activity and malondialdehyde (MDA) content were evaluated comprehensively, and salt tolerance was ranked according to the comprehensive evaluation value (D value) Which laid the foundation for the construction of alfalfa genetic population and the exploration of salt-related genes. The results showed that under the stress of 150 mmol·L -1 NaCl, the domestic alfalfa cultivars with ’Asi’> Xingping> Peru> Prairie No.3> (-1) Under NaCl stress, “Adina”> “giant” “giant can 7”> “drought 15 ”> “knight T ”. The salt and salt-sensitive germplasm resources were selected.