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运用最小二乘叠合技术及图象显示技术对已测定的不同种属和不同晶型的胰岛素及各种胰岛素衍生物空间结构进行了广泛的构象比较.研究结果表明:二锌胰岛素分子Ⅰ更接近于天然单体的结构;二体内两个分子之间的构象差异发生在二体形成过程中,并在六体形成及六体堆积过程中完善和稳定;通过一些铰链肽A10,B4,B8,B24,B20及B23,胰岛素分子内各结构片段之间具有较灵活的相对运动,羧端残基可能移动大于10A;由于所处的环境不同,各残基侧链的运动性差别很大.
The least square overlap technique and image display technique were used to conduct a wide range of conformational comparisons on the spatial structures of insulin and various insulin derivatives of different species and different crystal forms. The results show that the two zinc insulin molecules Ⅰ Close to the structure of the natural monomer; two body conformational differences between two molecules in the process of body formation, and in the six-body formation and six-body accumulation process of perfect and stable; through some of the hinge peptide A10, B4, B8 , B24, B20 and B23, the relative mobility among the structural segments of insulin molecules is relatively flexible, and the carboxyl terminal residues may move more than 10 A; due to the different environments, the mobility of the side chains of each residue varies greatly.