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目的:探讨清肝调脂饮对非酒精性脂肪性肝炎(NASH)大鼠脂质过氧化反应的干预机制。方法:Wistar大鼠70只,除10只作为正常对照组外,余60只建立以高脂饲料(基础饲料88%,猪油10%,胆固醇1.5%,胆盐0.5%)诱导的非酒精性脂肪性肝炎大鼠模型并随机分为5组,除模型组外,造模同时ig清肝调脂饮低、中、高剂量组(4.59,9.18,18.39 g.kg-1)和西利宾胺组(27 mg.kg-1,ig)分别按临床等效剂量的0.5,1,2和1倍剂量药物治疗。实验12周,大鼠全部处死。检测各组大鼠血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),甘油三酯(TG)含量,肝组织匀浆肿瘤坏死因子(TNF-α),丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性;肝组织行CD14,细胞色素P450 2E1(CYP2E1)免疫组化染色,阳性结果作定量分析。结果:与空白对照组比较,模型组ALT(62.57±17.08)U.L-1,TNF-α(902.00±22.66)pg.mg-1,CYP2E1阳性率(0.88±0.07),SOD(81.78±2.24)U.mg-1各指标均显示异常(P<0.05,P<0.01);与模型组比较,各用药组均能不同程度的改善以上各指标(P<0.05,P<0.01)。在降低TG,TNF-α方面,清肝调脂饮高剂量组明显优于西药对照组(P<0.01);而对其他各指标的改善,清肝调脂饮各剂量组与西药对照组疗效无显著差异。结论:清肝调脂饮具有降低脂质过氧化反应、提高抗氧化能力的作用,从而减轻NASH肝细胞变性坏死程度。
Objective: To investigate the mechanism of Qinggan tiaozhi decoction on lipid peroxidation in nonalcoholic steatohepatitis (NASH) rats. Methods: Seventy of Wistar rats were divided into four groups, except for 10 as normal control group, more than 60 mice were induced to establish non-alcoholic diet induced by high fat diet (88% basal diet, lard 10%, cholesterol 1.5%, bile salt 0.5%) The model rats with steatohepatitis were randomly divided into 5 groups. Except for the model group, the rats in the low, middle and high doses of ig qinggan tiaozhihu (4.59,9.18,18.39 g.kg-1) Group (27 mg.kg-1, ig) were clinically effective doses of 0.5, 1, 2 and 1 times the dose of drug treatment. All rats were sacrificed 12 weeks after the experiment. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), tumor necrosis factor-α (TNF- Content and superoxide dismutase (SOD) activity. The liver tissue was immunohistochemically stained for CD14 and CYP2E1, and the positive results were analyzed quantitatively. RESULTS: Compared with the blank control group, the positive rates of ALT (62.57 ± 17.08) UL-1, TNF-α (902.00 ± 22.66) pg.mg-1 and CYP2E1 in the model group were significantly higher than those in the blank control group (0.88 ± 0.07 and 81.78 ± 2.24) (P <0.05, P <0.01). Compared with the model group, all the above groups could improve the above indexes in different degrees (P <0.05, P <0.01). In reducing TG, TNF-α, the Qinggan Tiaozhi high dose group was significantly better than western medicine control group (P <0.01); while for other indicators of improvement, Qinggan Tiaozhi Yin each dose group and Western medicine control group No significant difference. Conclusion: Qinggan Tiaozhi Decoction can reduce the lipid peroxidation and improve the anti-oxidative capacity, thus reducing the degree of degeneration and necrosis of NASH hepatocytes.