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抽穗期是水稻重要的农艺性状之一,挖掘特异等位变异形式的抽穗期相关基因,对于改良水稻抽穗期至关重要。通过EMS诱变籼稻品种9311获得一个稳定遗传的早抽穗突变体ehd8。与野生型9311相比,ehd8在南宁自然长日照和南宁自然短日照条件下,分别提前抽穗6.7d和10.2d。此外,在南宁自然短日照条件下,ehd8的株高和每穗粒数比野生型9311显著降低。遗传分析表明,ehd8早抽穗性状受一对隐性核基因控制。EHD8基因被定位在8号染色体短臂上17kb范围内。基因注释信息表明,该定位区间内只有1个预测的候选基因LOC_Os080174500与已知的抽穗期基因EF8等位。序列比对分析发现,与野生型9311相比,ehd8突变体中LOC_Os080174500编码区的第11位碱基发生了G→T置换,造成其所编码蛋白的第四位氨基酸发生了精氨酸→蛋氨酸的置换。EHD8新等位基因的发掘对于丰富水稻特异种质资源以及促进其在水稻育种中的应用具有重要意义。
Heading date is one of the important agronomic traits in rice. It is very important to excavate the relevant genes of specific allelic variation at heading stage. A stable inheritance of early heading mutant ehd8 was obtained by EMS mutagenesis of indica rice 9311. Compared with the wild type 9311, ehd8 heading in the natural long days of Nanning and Nanning natural short day conditions, respectively, heading 6.7d and 10.2d. In addition, under natural short-day conditions in Nanning, ehd8 had significantly lower plant height and grains per spike than wild-type 9311. Genetic analysis showed that ehd8 early heading was controlled by a recessive nuclear gene. The EHD8 gene is located within 17 kb of the short arm of chromosome 8. Gene annotation information indicates that there is only one predicted candidate gene LOC_Os080174500 and the known heading date EF8 allele in the positioning interval. Sequence alignment analysis showed that compared with the wild type 9311, the substitution of G → T occurred in the 11th base of the LOC_Os080174500 coding region in the ehd8 mutant, resulting in the arginine → methionine at the fourth amino acid of the encoded protein The replacement. The discovery of new alleles of EHD8 is of great significance for enriching rice-specific germplasm resources and promoting its application in rice breeding.