纤维蛋白(原)在大鼠胸主动脉球囊成形术后肌内膜增殖中的作用

• 作 者: 刘京波 汪丽蕙 唐朝枢
• 机 构: 北京医科大学第一医院心内科,北京医科大学心血管基础研究所
• 出 处: 中国病理生理杂志
• 发表日期: 1999年01期
• 关键词: 内皮 血管 纤维蛋白原 肌 平滑

目的：探讨纤维蛋白（原）在大鼠胸主动脉球囊成形术后肌内膜增殖中的可能作用。方法：用生化方法检测术后血浆纤维蛋白原含量及用免疫组织化学方法。结果：纤维蛋白原含量在术后第１ｄ组大鼠（３８５±０３０）ｇ／Ｌ较假手术组大鼠（２４５±０２１）ｇ／Ｌ明显升高（Ｐ＜００１），第３ｄ组（６０８±０６８）ｇ／Ｌ又较第１ｄ组显著升高（Ｐ＜００１），第７ｄ组（５３７±１０５）ｇ／Ｌ较第３ｄ组无明显下降（Ｐ＞００５），第１４ｄ组（３８６±１０９）ｇ／Ｌ较第７ｄ组已明显下降（Ｐ＜００１），第２１ｄ组（３１０±０２６）ｇ／Ｌ较第１４ｄ组有所下降（Ｐ＜００５）。免疫组化研究发现术后１０ｍｉｎ，纤维蛋白（原）即在血管腔表面沉积，然后逐渐渗入增殖的内膜中。术后早期降低血浆纤维蛋白原的水平，可抑制血管壁３Ｈ－ＴｄＲ的掺入术后７ｄ，（２８９±１４７比１５８８±１８０）ｃｏｕｎｔｓ·ｍｉｎ－１／ｍｇ，Ｐ＜００１；术后１４ｄ，（２４２±７５比４６４±９１）ｃｏｕｎｔｓ·ｍｉｎ－１／ｍｇ，Ｐ＜００１）和肌内膜的增殖（内膜／中膜面积，０１２±００５比０３６±０１０，Ｐ＜００１；内膜／中膜厚度，０５６±０?

Objective: To investigate the possible role of fibrin (prot) in endometrial proliferation after thoracic aorta balloon angioplasty in rats. Methods: The biochemical methods were used to detect the content of fibrinogen and the immunohistochemical method. Results: The fibrinogen content in the first postoperative day (385 ± 030) g / L was significantly higher than that in sham operation group (245 ± 021) g / L (P < 0  01), 3d (6  08 ± 0  68) g / L was significantly higher than those in the 1d group (P <001), and in the 7th group (5  37 ± 1  05) g / L was significantly lower than that of the third day (P> 005), and the third day (386 ± 109) g / L was significantly lower than the seventh day (P <001) 3  10 ± 026) g / L decreased compared with the 14th group (P <0  05). Immunohistochemical study found that 10min after fibrin (original) that is deposited on the surface of the lumen, and then gradually infiltrated into the proliferation of the intima. In the early postoperative period, the level of plasma fibrinogen was decreased and the incorporation of 3H-TdR in blood vessel wall was inhibited 7 days after operation (289 ± 147 vs 1588 ± 180) counts · min-1 / mg, P <0.01; (242 ± 75 vs 464 ± 91) counts · min-1 / mg, P <001), and the proliferation of endomysia (intima / media area, 012 ± 005 to 036 ± 010, P <001; intima / media thickness, 056 ± 0?