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目的白介素-17(IL-17)作为前炎症因子可以导致趋化性细胞因子的增高,从而促进中性粒细胞和单核细胞的募集。本实验通过建立急性肺损伤(ALI)小鼠模型观察IL-17在体内环境下对中性粒细胞凋亡的影响,探讨体内环境下IL-17的变化与血液中性粒细胞的凋亡的关系。方法雄性C57小鼠30只,体质量22～27g,随机分为正常对照组(对照组),15只;ALI模型组(模型组),15只,脂多糖(LPS)腹腔注射6mg/kg、10ml/kg致急性肺损伤。分别观察小鼠呼吸频率、活动情况及直接光镜观察肺组织血管及肺泡变化情况,中性粒细胞凋亡,淋巴细胞CD3+/4+/8+分类检测,血清IL-17含量的检测。结果急性肺损伤时小鼠的IL-17的含量(0.10±0.002)pg/L,正常对照组(0.08±0.003)pg/L(P<0.05)。CD4+%模型组(21.99±1.83),对照组(14.97±0.99)(P<0.01)。中性粒细胞凋亡百分比(%)模型组(1.15±0.17)%,对照组(0.32±0.05)%(P<0.01)。结论在ALI发病24h时,由于各种炎性细胞因子及其他炎性介质的作用,中性粒细胞表达了较高的凋亡率,而IL-17在其中起到了一定作用,中性粒细胞的凋亡与IL-17有一定的相关性。
AIM Interleukin-17 (IL-17), as a proinflammatory cytokine, can lead to an increase in chemotactic cytokines, thereby promoting the recruitment of neutrophils and monocytes. In this study, the acute lung injury (ALI) mouse model was established to observe the effect of IL-17 on the neutrophil apoptosis in vivo and to explore the changes of IL-17 and the apoptosis of neutrophils in vivo relationship. Methods Thirty male C57 mice weighing 22-27 g were randomly divided into normal control group (n = 15), ALI model group (n = 15) and lipopolysaccharide (LPS) by intraperitoneal injection of 6 mg / kg, 10ml / kg induced acute lung injury. The respiration rate and activity of the mice were observed, the changes of the blood vessels and alveoli, the apoptosis of neutrophils, the detection of CD3 + / 4 + / 8 + lymphocytes and the level of IL-17 in the lung tissue were observed by light microscope. Results The content of IL-17 in mice with acute lung injury (0.10 ± 0.002) pg / L and normal control group (0.08 ± 0.003) pg / L (P <0.05). CD4 +% model group (21.99 ± 1.83), control group (14.97 ± 0.99) (P <0.01). The percentage of neutrophil apoptosis (%) in model group (1.15 ± 0.17)% and control group (0.32 ± 0.05)% (P <0.01). Conclusions At 24 hours after onset of ALI, neutrophils express higher rates of apoptosis due to various inflammatory cytokines and other inflammatory mediators, and IL-17 plays a role in them. Neutrophils Apoptosis and IL-17 have some relevance.