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目的观察平均功率密度为30 mW/cm2的连续微波辐射对大鼠学习记忆功能和海马葡萄糖摄取(glucose uptake)的影响及葡萄糖干预作用。方法成年雄性SD大鼠48只,其中18只用于Morris水迷宫实验,分为对照组、暴露组、葡萄糖干预暴露组,每组6只;暴露组、葡萄糖干预暴露组接受平均功率密度30 mW/cm2的微波连续暴露28 d,每天20 m in,暴露结束后Morris水迷宫检测大鼠学习记忆功能变化,Morris水迷宫训练前30 m in,葡萄糖干预暴露组大鼠按500 mg/kg腹腔注射葡萄糖,对照组和暴露组腹腔注射生理盐水。另30只用于海马葡萄糖摄取实验,分为对照组,暴露7、14、21、28 d组,每组6只,分别接受0、7、14、21、28 d平均功率密度为30 mW/cm2的微波暴露,各组暴露期满后,液体闪烁计数法检测大鼠海马组织对3H-2脱氧葡萄糖(3H-2DG)的摄取量变化。结果平均功率密度为30 mW/cm2的微波连续暴露28 d后,暴露组大鼠第4、5、6、7天逃避潜伏期较对照组延长(P<0.05),葡萄糖干预暴露组大鼠逃避潜伏期较对照组差异无显著性(P>0.05);暴露组大鼠在目标象限停留时间[(18.66±2.77)s]和跨越虚拟平台次数(1.60±0.54)均较对照组[(30.75±8.02)s,(2.80±0.83)]下降(P<0.05),葡萄糖干预暴露组大鼠目标象限停留时间[(26.84±4.75)s]和跨越虚拟平台次数(3.20±1.03)较对照组无显著性差异(P>0.05)。微波暴露21 d组和28 d组大鼠海马对3H-2-D-G lu-cose摄取量[(3 959±390),(3 764±192)]较对照组(5 284±711)显著减少(P<0.05)。结论腹腔注射葡萄糖可以改善微波暴露引起的学习记忆功能障碍,海马葡萄糖摄取减少可能是电磁辐射致学习记忆功能障碍的重要因素。
Objective To observe the effects of continuous microwave radiation with an average power density of 30 mW / cm2 on learning and memory ability and glucose uptake in the hippocampus and glucose intervention. METHODS: Forty-eight adult male Sprague-Dawley rats were randomly divided into control group, exposure group and glucose exposure group, with 18 rats in each group. Eighteen rats in the exposure group and glucose exposure group received average power density of 30 mW / cm2 microwave continuously for 28 days, daily for 20 mins. Morris water maze test was used to detect the learning and memory function of rats after exposure for 30 days. The Morris water maze training 30 m in before glucose exposure group was injected intraperitoneally with 500 mg / kg Glucose, control group and exposure group were intraperitoneally injected with saline. The other 30 rats were used for glucose uptake test in hippocampus and were divided into control group and exposed for 7, 14, 21 and 28 days, with 6 rats in each group. The average power density was 30 mW / cm2 microwave exposure. After the expiration of each group, the uptake of 3H-2-deoxyglucose (3H-2DG) in rat hippocampus tissue was measured by liquid scintillation counting. Results After exposure to microwave for 30 mW / cm2 for 28 days, the escape latency of rats in the exposure group was longer than that in the control group on the 4th, 5th, 6th and 7th days (P <0.05) (P <0.05). Compared with the control group (30.75 ± 8.02), the rats in the exposed group stayed in the target quadrant [(18.66 ± 2.77) s] and crossed the virtual platform (1.60 ± 0.54) s, (2.80 ± 0.83)] (P <0.05). There was no significant difference in target quadrant dwell time [(26.84 ± 4.75) s] and across virtual platform (3.20 ± 1.03) in glucose intervention group (P> 0.05). 3H-2-DG lu-cose uptake in the hippocampus of rats exposed to microwaves for 21 d and 28 d was significantly lower than that of the control group (5 284 ± 711 [(3 959 ± 390), (3764 ± 192)] P <0.05). Conclusion Intraperitoneal injection of glucose can improve learning and memory dysfunction caused by microwave exposure. The decrease of glucose uptake in hippocampus may be an important factor of learning and memory dysfunction caused by electromagnetic radiation.