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介绍了应用高效液相色谱-串联质谱(LC-MS/MS)与化学衍生化、在线氢/氘(H/D)交换技术联合研究粗提样品中药物代谢物结构的策略。讨论了子离子扫描、恒定中性丢失扫描、母离子扫描、多级的MSn和精确质量测量的质谱扫描技术。尤其重要的是,只需微量的粗提样品就足以满足LC-MS/MS的分析要求,且可以免去从复杂生物样品中纯化低含量代谢产物的困难,从而减少工作强度。
This paper introduces the strategy of combining HPLC-MS / MS with chemical derivatization and on-line hydrogen / deuterium (H / D) exchange technology to study the structure of drug metabolites in crude extracts. We discuss mass spectrometry techniques for sub-ion scans, constant neutral loss scans, precursor ion scans, multistage MSn, and accurate mass measurements. It is especially important that only a small amount of crude extract is sufficient to meet LC-MS / MS analysis requirements, eliminating the need to purify low levels of metabolites from complex biological samples and thereby reducing work intensity.