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目的 :研究回输自体PHA LAK细胞对恶性实体瘤患者淋巴细胞表型及功能状态的影响。方法 :入组2 0 4例实体瘤患者 ,并以 10例健康成人作为对照 ,采集与分离外周血单个核细胞 ,体外扩增PHA LAK细胞 ,采用流式细胞术分析淋巴细胞表型 ,MTT法检测淋巴细胞杀伤活性。结果 :实体瘤患者较正常人淋巴细胞亚群CD3 + /CD4+ 细胞、CD3 + /CD8+ 细胞、B细胞、NK细胞减少 ,淋巴细胞杀伤活性下降。扩增的PHA LAK是以激活的淋巴细胞亚群CD3 + /CD8+ 细胞、CD3 + /CD56+ 细胞、CD4+ /CD2 8+ 细胞为主的异质性群体 ,其数量及杀伤活性明显增加 ,激活淋巴细胞标记性抗原分子表达亦增加。PHA LAK细胞回输治疗后 ,患者外周血上述激活淋巴细胞亚群数量增加 ,淋巴细胞杀伤活性增强。结论 :实体瘤患者淋巴细胞亚群数量及功能低下 ,PHA LAK细胞过继性免疫治疗使患者体内激活淋巴细胞亚群数量增加 ,杀伤活性增强
OBJECTIVE: To study the effects of autologous PHA LAK cells translocation on lymphocyte phenotype and functional status in patients with malignant solid tumors. Methods: A total of 204 patients with solid tumors were enrolled and 10 healthy adults were taken as control. Peripheral blood mononuclear cells (PBMCs) were collected and isolated. PHA LAK cells were expanded in vitro. Lymphocyte phenotypes were analyzed by flow cytometry. MTT assay Detection of lymphocyte killing activity. Results: Compared with normal human lymphocytes, CD3 + / CD4 + cells, CD3 + / CD8 + cells, B cells and NK cells decreased in patients with solid tumors, and lymphocyte cytotoxicity decreased. The amplified PHA LAK is a heterogeneous population mainly composed of activated lymphocyte subsets CD3 + / CD8 + cells, CD3 + / CD56 + cells and CD4 + / CD2 + cells. The quantity and the cytotoxic activity of the expanded PHA LAK are significantly increased. The activated lymphocytes Marker antigen expression is also increased. After the PHA LAK cells were transfused, the number of activated lymphocyte subsets in the peripheral blood of patients increased and the lymphocyte cytotoxicity increased. CONCLUSIONS: The number and dysfunction of lymphocyte subsets in patients with solid tumors and the adoptive immunotherapy with PHA LAK cells increase the number of activated lymphocyte subsets and increase the cytotoxic activity