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为了解ＨＩＶ抗体阳性血浆中的ＨＩＶ１病毒基因亚型的情况，应用逆转录ＰＣＲ和ＤＮＡ序列测定技术，对６份获自高危人群的抗ＨＩＶ１阳性血浆进行序列分析和基因亚型分型的研究，结果表明均属ＨＩＶ１Ｂ亚型。Ｖ３环氨基酸序列分析指出这些ＨＩＶ１Ｂ亚型病毒株与泰国ＨＩＶ１Ｂ亚型病毒株核苷酸和氨基酸序列相似；同时发现ＨＩＶ１ｃＤＮＡ和氨基酸序列均相同，推测这６份标本可能来自同时感染同一株ＨＩＶ病毒的感染者。本研究对了解高危人群中ＨＩＶ１流行的遗传变异和ＨＩＶ１亚型病毒株的分子流行病分析具有一定的意义。
In order to understand the HIV-1 HIV subtype in HIV-positive plasma, RT-PCR and DNA sequencing were used to analyze the sequence of 6 anti-HIV-1 positive plasma from high-risk groups and to analyze the gene subtypes Type of research, the results show that are HIV 1B subtype. V3 loop amino acid sequence analysis pointed out that these HIV subtype 1B subtype strains and Thailand HIV subtype 1B subtype virus nucleotide and amino acid sequence similarity; also found HIV 1 cDNA and amino acid sequence are the same, suggesting that the six specimens may come from the same time Infected with the same HIV infection. This study is of great significance to understand the genetic variation of HIV-1 prevalence in high-risk groups and the molecular epidemiological analysis of HIV-1 subtypes.