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目的:探讨CD4+T淋巴细胞内三磷酸腺苷(adenosine triphosphate,ATP)含量与膀胱癌术后复发的关系。方法:本研究采集67例膀胱癌患者(非复发53例,复发14例)及53名健康志愿者的外周血标本,用ImmuKnowTM免疫细胞功能测定试剂盒检测CD4+T淋巴细胞内ATP值。结果:健康对照组、非复发组、复发组的CD4+T淋巴细胞内ATP含量分别为(449±116)ng/ml(、298±101)ng/ml(、141±98)ng/ml;复发组和非复发组的CD4+T淋巴细胞内ATP值显著低于健康对照组(P<0.01),复发组的ATP含量显著低于非复发组(P<0.01),ATP值降低(<142ng/ml)时膀胱癌复发的风险增加。结论:CD4+T淋巴细胞内ATP含量与膀胱癌术后复发有良好相关性,监测膀胱癌术后患者的上述指标对评估其免疫状态、制定个体化治疗方案,预防复发具有良好的指导作用。
Objective: To investigate the relationship between the content of adenosine triphosphate (ATP) in CD4 + T lymphocytes and the recurrence of bladder cancer. Methods: Peripheral blood samples from 67 patients with bladder cancer (53 non-recurrent cases, 14 recurrent cases) and 53 healthy volunteers were collected and the ATP value in CD4 + T lymphocytes was detected by ImmuKnowTM immune cell functional assay kit. Results: The levels of ATP in CD4 + T lymphocytes were (449 ± 116) ng / ml (298 ± 101) ng / ml (141 ± 98) ng / ml in healthy control group, non - recurrent group and relapsed group, The ATP value of CD4 + T lymphocytes in relapsed and non-relapsed patients was significantly lower than that in healthy controls (P <0.01). The ATP content in relapsed patients was significantly lower than that in non-relapsed patients (P <0.01) / ml) increased the risk of bladder cancer recurrence. CONCLUSION: The content of ATP in CD4 + T lymphocytes has a good correlation with the postoperative recurrence of bladder cancer. Monitoring the above indexes in postoperative patients with bladder cancer has a good guiding role in assessing the immune status, developing individualized treatment plan and preventing relapse.