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目的为探讨恶性纤维组织细胞瘤的增殖细胞核抗原（PCNA）及核仁组成区嗜银蛋白（AgNOR）定量检测与肿瘤恶性度及预后的关系。方法应用免疫组织化学杂色LSAB法及AgNOR染色法对46例资料完整有随访结果的恶性纤维组织细胞瘤（MFH）手术切除标本进行染色以显示PCNA及AgNOR，彩色病理图像分析系统定量检测。结果肿瘤细胞核PCNA过表达及AgNOR计数与患者生存期呈负相关（rPCNA＝－0．6248、rAgNOR＝－0．5941，P＜0.01）；PCNA及AgNOR定量高值组较之定量低值组患者生存期短（P＜0．01）；肿瘤有转移组PCNA及AgNOR定量高于无转移组（P＜0．01）；肿瘤位置深在组PCNA及AgNOR定量高于肿瘤位置浅表组（P＜0．01）。结论肿瘤细胞核PCNA及AgNOR定量与患者生存期密切相关，与肿瘤转移及位置深浅等反映肿瘤恶性度的指标亦密切相关，提示肿瘤细胞核PCNA及AgNOR定量对于判断恶性纤维组织细胞瘤恶性度及预后的意义值得重视。
Objective To investigate the relationship between the quantitative detection of proliferating cell nuclear antigen (PCNA) and argentinary arginine (AgNOR) in malignant fibrous histiocytoma and its relationship with malignancy and prognosis. Methods Immunohistochemistry LSAB and AgNOR staining methods were used to stain 46 specimens of malignant fibrous histiocytoma (MFH) with intact follow-up results to show PCNA and AgNOR. The color pathological image analysis system was used for quantitative detection. Results There was a negative correlation between PCNA overexpression and AgNOR counts in the tumor cell nuclei and survival time (rPCNA=-0.6248, rAgNOR=-0.5941, P<0.01); the PCNA and AgNOR quantitative high value group was lower than the quantitative low value group. The survival time was short (P<0.01); the quantification of PCNA and AgNOR in the tumor metastasis group was higher than that in the non-metastasis group (P<0.01); the tumor location was deeper in the group; the PCNA and AgNOR quantification was higher than the tumor location in the superficial group (P <0.01). Conclusion The quantification of PCNA and AgNOR in tumor cell nuclei is closely related to the survival time of patients. It is also closely related to the indicators of tumor metastasis and location depth, which reflect the degree of tumor malignancy. It suggests that the quantification of PCNA and AgNOR in tumor nuclei can be used to judge the malignancy and prognosis of malignant fibrous histiocytoma. Meaning is worth paying attention to.