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以6份青稞品系为供试材料,研究提取和预处理过程中在提取液中添加ABA和秋水仙碱对小孢子活力的影响,以及诱导培养基中添加不同浓度亚精胺、2,4-D和麦芽糖对愈伤组织诱导和绿苗分化产量的影响。结果表明在提取和预处理过程中提取液中添加秋水仙碱均可以提高青稞小孢子活力,诱导培养基中添加亚精胺可以提高愈伤组织诱导和绿苗分化产量,愈伤组织诱导培养基中2,4-D 1.5~2.0 mg·L-1和麦芽糖60~90 g·L-1浓度比较适合青稞小孢子的培养。
Six barley varieties were used as materials to study the effects of adding ABA and colchicine on the microspore activity during the extraction and pretreatment. The effects of different concentrations of spermidine and 2,4- Effect of D and maltose on callus induction and green shoot differentiation. The results showed that during the extraction and pretreatment, the addition of colchicine could improve the microspore vitality of callus, and the addition of spermidine to the induction medium could increase the callus induction and green shoot differentiation yield, callus induction medium The concentrations of 2,4-D 1.5-2.0 mg · L-1 and maltose 60-90 g · L-1 were suitable for the culture of microspore.