肺癌组织的P53基因突变与细胞增殖———P53与增殖细胞核抗原免疫组化研究

• 作 者: 喻伦银 刘铭球 邹祖玉 江曼 张正彬
• 出 处: 湖北医科大学学报
• 发表日期: 1996年03期
• 关键词: P53基因 增殖细胞核抗原 免疫组化研究 细胞增殖 肺肿瘤 组织学技术 细胞生长 肺鳞癌 抗核因子 肺腺癌

运用免疫组织化学技术，以Ｐ５３基因蛋白和增殖细胞核抗原（ＰＣＮＡ）表达为指标，探讨了人肺癌组织中的Ｐ５３基因突变与细胞增殖的关系。结果表明：癌组织中的Ｐ５３基因突变与肿瘤细胞的增殖活性呈正相关。表明突变型Ｐ５３有促进细胞生长的作用，为阐明Ｐ５３基因在细胞生长调控中的作用增添了实验依据。

Using immunohistochemical techniques, the expression of P53 gene protein and proliferating cell nuclear antigen (PCNA) were used as indicators to investigate the relationship between P53 gene mutation and cell proliferation in human lung cancer tissues. The results showed that the mutation of P53 gene in cancer tissues was positively correlated with the proliferation activity of tumor cells. It was suggested that mutant P53 could promote cell growth, which provided experimental basis for elucidating the role of P53 in the regulation of cell growth.