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以纯纤维素为底物,考察了阳离子表面活性剂1-十六烷基吡啶氯盐对纯纤维素酶解糖化的优化条件及其对体系酶活和酶动力学的影响。结果表明:当酶量为10 FPU/g,底物质量分数为1.25%,1-十六烷基吡啶氯盐质量浓度为5 g/L,反应时间为12 h时,纤维素平均转化率为59.91%,比不添加表面活性剂的空白样转化率提高了20.79%。1-十六烷基吡啶氯盐的加入,提高了酶解反应的最大反应速率和米氏常数,其中,最大反应速率提高了58.22%,米氏常数提高了101.42%。相对酶活提高率随时间呈现先升高后降低的规律,在24 h时,相对酶活提高率最高值达53.18%。
Using pure cellulose as substrate, the optimum conditions for the enzymatic hydrolysis and saccharification of 1-hexadecylpyridinium chloride (CTA) and its effect on enzyme activity and enzyme kinetics were investigated. The results showed that when the amount of enzyme was 10 FPU / g, the mass fraction of substrate was 1.25%, the mass concentration of 1-hexadecyl pyridinium chloride was 5 g / L and the reaction time was 12 h, the average conversion of cellulose was 59.91%, which is 20.79% higher than the blank-like conversion without surfactant addition. The addition of 1-hexadecylpyridinium chloride increased the maximum reaction rate and the Michaelis constant of the enzymatic reaction, in which the maximum reaction rate increased 58.22% and the Michaelis constant increased by 101.42%. The rate of relative enzyme activity increased first and then decreased with time, and the highest rate of relative enzyme activity reached 53.18% at 24 h.