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目的山奈酚是一种有抗炎、抗氧化作用的黄酮类化合物。前期研究发现,该化合物在体外水平可抑制膀胱癌化疗药顺铂诱导的心肌细胞凋亡。本研究主要在体内条件下探讨山奈酚对顺铂心脏毒性的抑制作用。方法实验分为对照组、顺铂组、顺铂+山奈酚(50 mg/kg)组和顺铂+山奈酚(150 mg/kg)组,每组5只大鼠,采用腹腔给药处理14天。之后,分别检测心肌损伤指标LDH、CK-MB、cTnⅠ及cTnT的变化,氧化应激指标MDA、GSH和SOD的表达变化。通过ELISA检测炎症因子TNF-α的表达水平。结果在相应处理后14天,发现顺铂处理组血清LDH、CK-MB、cTnⅠ及cTnT,心脏氧化应激标志物MDA、GSH和SOD以及炎症因子TNF-α的表达水平较对照组均显著升高。而同时给予山奈酚处理的大鼠上述指标均较单纯顺铂处理组下调,且较高剂量山奈酚(150 mg/kg)组抑制作用更明显。结论山奈酚可在体内水平抑制顺铂诱导的心肌损伤。
The purpose of kaempferol is a kind of anti-inflammatory, anti-oxidant flavonoids. Preliminary studies have found that the compound can inhibit cisplatin-induced cardiomyocyte apoptosis in vitro in vitro. This study mainly in vivo conditions kaempferol on the inhibition of cisplatin cardiotoxicity. Methods The experiment was divided into control group, cisplatin group, cisplatin + kaempferol (50 mg / kg) group and cisplatin + kaempferol (150 mg / kg) day. Afterwards, the change of myocardial injury index LDH, CK-MB, cTnⅠ and cTnT, the changes of MDA, GSH and SOD were detected respectively. The expression level of inflammatory cytokine TNF-α was detected by ELISA. Results The serum levels of LDH, CK-MB, cTnI and cTnT, cardiac oxidative stress markers MDA, GSH and SOD as well as inflammatory cytokines TNF-α in cisplatin-treated group were significantly higher than those in control group high. The above indexes of rats treated with kaempferol were all lower than that of the cisplatin-treated group, and the inhibitory effect of the kaempferol (150 mg / kg) was more obvious. Conclusion Kaempferol can inhibit cisplatin-induced myocardial injury in vivo.