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目的：研究昆明山海棠对人白血病细胞ＨＬ－６０ＨＰＲＴ位点的影响。方法：用ＴＨＨ水溶液对ＨＬ－６０细胞进行浓度梯度染毒，不同时间点进行单细胞微孔接种，计数阳性克隆，测定接种存活率、克隆效率和突变频率。结果：ＴＨＨ染毒细胞的突变频率，在处理剂量３．３５～２０．１０ｍｇ／ｍｌ范围内，具有明显的剂量－反应关系。但随着ＴＨＨ的浓度增高至３３．５０ｍｇ／ｍｌ组，由于细胞存活率太低，细胞数不够最后接种要求，不能继续进行。这一现象可能与ＴＨＨ的细胞毒性有关。结论：ＴＨＨ对ＨＬ－６０细胞具有肯定的致突变性，但只是在一定的剂量范围内遵循剂量反应规律
Objective: To study the effect of Kunming Mountain Begonia on the HL-60 HPRT locus of human leukemia cells. Methods: HL-60 cells were treated with THH aqueous solution in concentration gradient. Single cell micropore inoculation was performed at different time points. Positive clones were counted and the survival rate, cloning efficiency and mutation frequency were determined. RESULTS: The frequency of mutation of THH-treated cells was in the range of 3.35 to 20.10 mg/ml. There was a clear dose-response relationship. However, as the concentration of THH increased to 33.50 mg/ml, the cell survival rate was too low and the number of cells was not enough for the final vaccination request and could not be continued. This phenomenon may be related to the cytotoxicity of THH. Conclusion: THH has a certain mutagenicity on HL-60 cells, but only following a dose-response rule within a certain dose range.