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目的 研究胃癌患者外周血中sCD44v6含量及其组织中CD44v6蛋白表达 ,探讨sCD44v6含量 /CD44v6蛋白与临床病理参数之间的关系。方法 应用酶联免疫吸附试验 (ELISA)检测胃癌患者 (术前 )及其中部分术后及健康对照组血清中sCD44v6含量并以 (S P)免疫组织化学法测定相应组织中CD44v6蛋白的表达。结果 70例胃癌患者血清中sCD44v6含量 (2 .15± 0 .78)μg/L明显高于 16例正常对照组 (1.18± 0 .43 ) μg/L、14例根治性手术后血清中sCD44v6含量(1.2 1± 0 .3 9) μg/L比术前 (2 .67± 0 .83 ) μg/L明显下降 (P <0 .0 1) ,而 6例非根治性手术后(3 .2 9± 0 .41) μg/L比术前 (3 .61± 0 .49) μg/L下降不明显 (P >0 .0 5 )。结论 胃癌患者外周血中sCD44v6含量及组织中CD44v6蛋白表达的变化与转移、临床分期、病理分期有关 ,sCD44v6含量升高可作为胃癌患者淋巴结转移 (尤其是早期转移 )的监测指标。
Objective To study the expression of sCD44v6 in peripheral blood and the expression of CD44v6 protein in gastric cancer patients and to explore the relationship between sCD44v6 protein / CD44v6 protein and clinicopathological parameters. Methods Serum levels of sCD44v6 in gastric cancer patients (preoperative) and some postoperative and healthy controls were detected by enzyme linked immunosorbent assay (ELISA), and the expression of CD44v6 protein in corresponding tissues was detected by (SP) immunohistochemistry. Results The serum levels of sCD44v6 (2.15 ± 0.78) μg / L in 70 patients with gastric cancer were significantly higher than those in 16 healthy controls (1.18 ± 0.43) μg / L and 14 patients with postoperative radical surgery (1.2 ± 0.39) μg / L decreased from 2.67 ± 0.83 μg / L preoperatively (P <0.01) 9 ± 0.41) μg / L compared with preoperative (3.61 ± 0.49) μg / L was not significantly decreased (P> 0.05). Conclusion The changes of sCD44v6 and the expression of CD44v6 in peripheral blood of patients with gastric cancer are related to the metastasis, clinical stage and pathological stage. The increased sCD44v6 may be used as the indicator of lymph node metastasis (especially early metastasis) in patients with gastric cancer.