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用反转录－多聚酶链反应（ＲＴ－ＰＣＲ）检测汉坦病毒（Ｈａｎｔａｖｉｒｕｓ）的基因ＲＮＡ，同时与细胞分离病毒的敏感性进行比较。用异硫氰酸胍－载体吸附法，一步提取汉坦病毒ＲＮＡ。将脑腔接种汉坦病毒频临死亡的乳鼠脑制成１０％的悬液，并系列稀释，从１０（－１）到１０（－１２）的１２梯度。结果表明，该引物能够扩增出汉坦病毒Ｈ８２０５株的ＲＮＡ，说明汉坦病毒Ｈ８２０５株与７６－１１８株在Ｍ片段上具有共同的保守序列。ＲＴ－ＰＣＲ可以检测出４．６４×１０（－３）ＴＣＬＤ（５０）／ｍｌ的病毒，而用Ｖｅｒｏ－Ｅ６细胞只能分离检测出４．６４×１０（－２）ＴＣＬＤ（５０）／ｍｌ，所以ＲＴ－ＰＣＲ检测病毒比细胞分离检测病毒敏感。
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the gene RNA of Hantavirus, and compared with the sensitivity of cells to virus isolation. Hantaan virus RNA was extracted in one step by guanidine isothiocyanate-carrier adsorption method. Brains were challenged with 10% suspension of neonatal hantavirus brain and in serial dilutions ranging from 10 (-1) to 10 (-12) in 12 gradients. The results showed that this primer could amplify the RNA of Hantavirus H8205 strain, indicating that Hantavirus H8205 strain and 76-118 strain shared a common conserved sequence in M fragment. RT-PCR detected 4.64 × 10 (-3) TCLD (50) / ml of virus, whereas only 4.64 × 10 (-2) TCLD (50) / ml was detected in Vero-E6 cells , So RT-PCR detection of virus than cell-based detection of virus-sensitive.