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[目的]观察900MHz微波辐射对雄性大鼠血浆褪黑素、睾酮和雌二醇水平昼夜节律的影响。[方法]健康雄性SD大鼠30只,适应性喂养30d后采集大鼠24h自发活动数据,利用生物节律单余弦分析软件筛选具有昼夜节律的大鼠17只,再对该17只大鼠24h采血6次测血浆褪黑素水平,然后选取褪黑素水平昼夜节律差异无统计学意义的大鼠10只,随机分为对照组和照射组,每组5只。照射组在昼夜时点(ZT)1点给予2000μW/cm2900MHz的微波辐射,1h/d,持续30d;对照组除不给予微波辐射外,其他条件同照射组(即假照射)。大鼠在辐射结束后第0、4、8、12、16、20h各采血一次,测定大鼠血浆的褪黑素、睾酮和雌二醇水平,比较照射组与对照组血浆褪黑素、睾酮和雌二醇水平昼夜节律的不同。[结果]对照组在假照射后血浆褪黑素水平、睾酮水平存在明显昼夜节律,照射组在微波辐射后血浆褪黑素水平和睾酮水平也存在明显昼夜节律,但微波辐射后相位响应曲线的中值、振幅、峰值时点与对照组假照射后相比发生了明显变化(P<0.05)。对照组假照射后血浆雌二醇水平有明显昼夜节律(P<0.05),照射组微波辐射后血浆雌二醇水平不存在昼夜节律(P>0.05)。[结论]2000μW/cm2900MHz微波辐射可影响雄性大鼠血浆褪黑素、睾酮和雌二醇水平的昼夜节律。
[Objective] To observe the effect of 900MHz microwave radiation on the circadian rhythm of melatonin, testosterone and estradiol in male rats. [Methods] Thirty healthy male Sprague-Dawley rats were randomly selected. After 30 days of adaptive feeding, spontaneous activity data of rats were collected for 24 hours. Seventeen circadian rhythms were screened by single cosine analysis software. 6 times to measure the level of plasma melatonin, and then select the melatonin level of circadian rhythms in 10 non-statistically significant rats were randomly divided into control group and irradiation group, 5 in each group. The irradiation group was given microwave irradiation of 2000μW / cm at 2900MHz at 1 o’clock of day and night for 1h / d for 30 days. The control group was given the same conditions as the irradiation group except the microwave irradiation. Rats were harvested once on the 4th, 4th, 12th, 16th, 20th and 20th hour after irradiation. The levels of melatonin, testosterone and estradiol in the plasma of the rats were measured. The levels of melatonin, testosterone And the difference of circadian rhythm of estradiol level. [Result] The control group had obvious circadian rhythm of plasma melatonin level and testosterone level after the sham irradiation. There was also a significant circadian rhythm of plasma melatonin level and testosterone level in the irradiated group after microwave irradiation. However, the phase response curve after microwave irradiation Median, amplitude, peak time point compared with the control group after irradiation had a significant change (P <0.05). There was a significant circadian rhythm of plasma estradiol level in the control group after irradiation (P <0.05). There was no circadian rhythm (P> 0.05) in plasma estradiol levels after irradiation in the control group. [Conclusion] The diurnal rhythm of plasma melatonin, testosterone and estradiol levels in male rats may be affected by microwave radiation at 2000μW / cm 2900MHz.