茎直黄芪中苦马豆素的提取工艺研究

• 作 者: 郝宝成 杨贤鹏 王学红 陈慧 郭文柱 郭志廷 刘宇 尚若锋 胡永浩 梁剑平
• 机 构: 中国农业科学院兰州畜牧与兽药研究所/农业部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室,甘肃农业大学动物医学院,
• 出 处: 湖北农业科学
• 发表日期: 2013年08期
• 关键词: 茎直黄芪 苦马豆素 提取率 酶法提取 工艺研究 正交试验 纤维素酶 工艺条件 Astragalus 气相色谱法

以茎直黄芪(Astragalus strictus)为原料,通过单因素试验、正交试验和方差分析确定了苦马豆素的酶法提取工艺条件,并用气相色谱法测定了提取物中苦马豆素的含量。结果表明,优化后的茎直黄芪中苦马豆素最佳提取条件为,粉碎目数80目、料液比1∶40、纤维素酶添加量3.5%、酶解时间3.0 h。在此提取条件下,苦马豆素的提取率可达到39.41 mg/kg。该法快速、高效、方便、节能,有利于茎直黄芪中苦马豆素的提取。

Using Astragalus strictus as raw material, the enzymatic extraction conditions of swainine were determined by single factor test, orthogonal test and analysis of variance. The content of swainsonine in the extract was determined by gas chromatography . The results showed that the optimal extraction conditions of sow malachovin in the optimized Astragalus membranaceus were as follows: smash mesh 80 meshes, solid-liquid ratio 1:40, cellulase addition 3.5%, enzymolysis time 3.0 h. Under this extraction condition, the extraction yield of swainsonine reached 39.41 mg / kg. The method is fast, efficient, convenient, energy saving, is conducive to the extraction of sow vetiver in Astragalus.